Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,137	C→G	A289P (GCG→CCG)	rclA ←	putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,137	0	C	G	100.0%	130.0 / NA	38	A289P (GCG→CCG)	rclA	putative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/19);  total (19/19)

TAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCA  >  NC_000913/319060‑319213                                                                              |                                                                             tAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCa                                                                           <  2:73134/81‑1 (MQ=255) tAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCa                                                                           <  2:73133/81‑1 (MQ=255)          atgTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTc                                                                  >  2:73171/3‑81 (MQ=255)            gTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTg                                                                >  1:73167/1‑81 (MQ=255)               cAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCgg                                                             <  1:73136/81‑1 (MQ=255)               cAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCg                                                              <  2:73135/80‑1 (MQ=255)                 aTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCggtg                                                           >  1:73166/1‑81 (MQ=255)                  tGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCggtgg                                                          <  2:73137/81‑1 (MQ=255)                     ggTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTAt                                                       >  2:73165/1‑81 (MQ=255)                        aaTCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGt                                                     >  1:73181/1‑80 (MQ=255)                         atcatcCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTaa                                                   >  1:73163/1‑81 (MQ=255)                            atcCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATc                                                 <  2:73138/80‑1 (MQ=255)                              ccAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCt                                              >  1:73185/1‑81 (MQ=255)                               cAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCtt                                             <  2:73139/81‑1 (MQ=255)                                     tatGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAAc                                       <  2:73140/81‑1 (MQ=255)                                      atGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAAc                                       >  2:73175/1‑80 (MQ=255)                                      atGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAAc                                       >  2:73204/1‑80 (MQ=255)                                        gtaagtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGa                                     >  2:73227/1‑80 (MQ=255)                                         taagtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGAc                                    <  2:73141/80‑1 (MQ=255)                                          aagtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACaa                                  >  1:73156/1‑81 (MQ=255)                                           agtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACaa                                  <  1:73143/80‑1 (MQ=255)                                           agtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAAt                                 <  2:73142/81‑1 (MQ=255)                                            gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAAtt                                <  1:73144/81‑1 (MQ=255)                                            gtaaATTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAAtt                                >  2:73170/1‑81 (MQ=255)                                               aaTTGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGc                              <  2:73145/80‑1 (MQ=255)                                                 ttGCAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGcccc                           <  2:73146/81‑1 (MQ=255)                                                    cAGCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCcgcg                        <  2:73147/81‑1 (MQ=255)                                                      gCCCGCCGGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCt                      <  1:73148/81‑1 (MQ=255)                                                        ccgccgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCg                    >  2:73172/1‑81 (MQ=255)                                                          gccgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGtt                  >  1:73178/1‑81 (MQ=255)                                                          gccgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGtt                  >  2:73183/1‑81 (MQ=255)                                                            cgGTAACATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTa                >  1:73201/1‑81 (MQ=255)                                                                aaCATCTCCCATCGGCCAAATATTGTTTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGc            >  1:73164/1‑81 (MQ=255)                                                                aaCATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGc            >  1:73186/1‑81 (MQ=255)                                                                 aCATCTCCCATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCg           <  2:73149/81‑1 (MQ=255)                                                                        ccATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCgg    <  1:73150/81‑1 (MQ=255)                                                                        ccATCGGCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCgg    <  1:73151/81‑1 (MQ=255)                                                                          aTCGGCCAAATATTGTCTGCGGTGGTATGTAATCGATTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCa  >  2:73190/1‑81 (MQ=255)                                                                              |                                                                             TAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCGCCGGTAACATCTCCCATCGCCCAAATATTGTCTGCGGTGGTATGTAATCGCTTGTCAACGACAATTGCCCCGCGCTCGTTTACTGCGATACCGGCA  >  NC_000913/319060‑319213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑