Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,152,454	A→G	E73E (GAA→GAG)	metC →	cystathionine beta‑lyase/L‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,152,454	0	A	G	100.0%	153.1 / NA	44	E73E (GAA→GAG)	metC	cystathionine beta‑lyase/L‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

AATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAACTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCTTTTATCG  >  NC_000913/3152377‑3152530                                                                              |                                                                             aaTCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTg                                                                           >  1:583642/1‑81 (MQ=255)  aTCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTg                                                                           <  1:583595/80‑1 (MQ=255)   tCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTgg                                                                          >  1:583630/1‑80 (MQ=255)         cAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTgg                                                                   <  1:583596/81‑1 (MQ=255)          aaTGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGgc                                                                  >  1:583643/1‑81 (MQ=255)                      ttCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTg                                                      <  1:583597/81‑1 (MQ=255)                       tCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGc                                                     >  1:583637/1‑81 (MQ=255)                        cTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGc                                                     <  1:583598/80‑1 (MQ=255)                             gACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTc                                               >  2:583660/1‑81 (MQ=255)                             gACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTc                                               >  1:583639/1‑81 (MQ=255)                              aCGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTcc                                              <  2:583599/81‑1 (MQ=255)                              aCGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTcc                                              >  1:583662/1‑81 (MQ=255)                               cGGCACGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTccc                                             <  1:583600/81‑1 (MQ=255)                                ggCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCt                                            >  2:583654/1‑81 (MQ=255)                                  cgcgGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGc                                          >  2:583646/1‑81 (MQ=255)                                  cgcgGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGc                                          <  2:583601/81‑1 (MQ=255)                                     ggAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCggg                                       >  1:583668/1‑81 (MQ=255)                                     ggAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCggg                                       <  1:583602/81‑1 (MQ=255)                                       aaCGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGggc                                     <  1:583603/81‑1 (MQ=255)                                       aaCGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGggc                                     <  2:583604/81‑1 (MQ=255)                                          gTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGggcggc                                  <  2:583605/81‑1 (MQ=255)                                          gTTAACCCATTTCTCCTTACAACAAGCGATGAGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGggcggc                                  <  2:583606/81‑1 (MQ=255)                                              aCCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGc                               <  1:583607/80‑1 (MQ=255)                                              aCCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGc                               <  2:583609/80‑1 (MQ=255)                                              aCCCATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGc                               <  1:583608/80‑1 (MQ=255)                                                ccATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGt                            >  2:583644/1‑81 (MQ=255)                                                 cATTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGtt                           <  2:583610/81‑1 (MQ=255)                                                  aTTTCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTg                          <  2:583611/81‑1 (MQ=255)                                                   tttCTCCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGc                         >  1:583649/1‑81 (MQ=255)                                                     tctcCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCt                        <  2:583612/80‑1 (MQ=255)                                                     tctcCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCt                        <  2:583613/80‑1 (MQ=255)                                                       tcCTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAAt                     >  2:583661/1‑81 (MQ=255)                                                         cTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAAtt                    <  1:583615/80‑1 (MQ=255)                                                         cTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAAtt                    <  2:583614/80‑1 (MQ=255)                                                         cTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTc                   >  2:583651/1‑81 (MQ=255)                                                         cTTACAACAAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTc                   >  2:583655/1‑81 (MQ=255)                                                            acaacaAGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCAt                >  2:583628/1‑81 (MQ=255)                                                              aacaaGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGTGGCAGCGGTTGCTAATTCCATTc              >  1:583647/1‑81 (MQ=255)                                                              aacaaGCGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTc              <  1:583616/81‑1 (MQ=255)                                                                    cGATGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCtt        >  1:583682/1‑81 (MQ=255)                                                                      aTGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCtttt      <  1:583617/81‑1 (MQ=255)                                                                      aTGTGTGAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCttt       >  1:583666/1‑80 (MQ=255)                                                                          gtgAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCTTTTATc   >  2:583663/1‑80 (MQ=255)                                                                           tgAGCTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCTTTTATCg  >  1:583670/1‑80 (MQ=255)                                                                              |                                                                             AATCGCGCCAATGGAGAGTTGTTCTATGGACGGCGCGGAACGTTAACCCATTTCTCCTTACAACAAGCGATGTGTGAACTGGAAGGTGGCGCAGGCTGCGTGCTATTTCCCTGCGGGGCGGCAGCGGTTGCTAATTCCATTCTTGCTTTTATCG  >  NC_000913/3152377‑3152530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑