Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,351	T→C	N39S (AAC→AGC)	ribB ←	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,351	0	T	C	100.0%	119.8 / NA	35	N39S (AAC→AGC)	ribB	3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

CCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTC  >  NC_000913/3184276‑3184427                                                                            |                                                                             ccGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTc                                                                          <  1:591464/80‑1 (MQ=255)    cTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATTATATCACCTTCGCTTTCACgg                                                                      >  2:591496/1‑81 (MQ=255)     tACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGt                                                                     >  2:591523/1‑81 (MQ=255)      aCCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTc                                                                    >  2:591530/1‑81 (MQ=255)       ccGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTc                                                                    <  1:591465/80‑1 (MQ=255)       ccGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCt                                                                   <  1:591466/81‑1 (MQ=255)         gTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtc                                                                 <  2:591467/81‑1 (MQ=255)            gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcatc                                                              <  1:591469/81‑1 (MQ=255)            gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcat                                                               <  1:591468/80‑1 (MQ=255)            gCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcat                                                               >  2:591519/1‑80 (MQ=255)                aTGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTtcatcatc                                                           >  1:591497/1‑80 (MQ=255)                 tGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCaa                                                         <  1:591470/81‑1 (MQ=255)                 tGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCaa                                                         <  2:591471/81‑1 (MQ=255)                      aGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCAc                                                     >  2:591522/1‑80 (MQ=255)                          ccATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTa                                                >  1:591521/1‑81 (MQ=255)                           cATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTac                                               <  1:591472/81‑1 (MQ=255)                            aTCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTaca                                              <  2:591473/81‑1 (MQ=255)                                 cTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACAcc                                            <  1:591474/78‑1 (MQ=255)                                    aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGt                                        <  1:591476/79‑1 (MQ=255)                                    aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTc                                       <  1:591477/80‑1 (MQ=255)                                    aaCAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTc                                       <  1:591475/80‑1 (MQ=255)                                      cAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCtt                                    <  2:591478/81‑1 (MQ=255)                                         tCATGGTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAc                                 <  2:591479/81‑1 (MQ=255)                                              gTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagc                            <  1:591480/81‑1 (MQ=255)                                              gTTTCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagc                            <  1:591481/81‑1 (MQ=255)                                                ttCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcg                           >  1:591527/1‑80 (MQ=255)                                                 tCTGCCGGGAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCAcgcagcgc                          <  1:591482/80‑1 (MQ=255)                                                       gggAAGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGt                   >  2:591544/1‑81 (MQ=255)                                                          aaGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCa                >  2:591535/1‑81 (MQ=255)                                                           aGATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAt               >  1:591541/1‑81 (MQ=255)                                                            gATCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAtt              >  2:591545/1‑81 (MQ=255)                                                             aTCATATCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCAttt             >  1:591526/1‑81 (MQ=255)                                                                   tCACCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAac        <  2:591483/80‑1 (MQ=255)                                                                     aCCTTCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACg     <  1:591484/81‑1 (MQ=255)                                                                         tCGCTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTc  >  2:591529/1‑80 (MQ=255)                                                                            |                                                                             CCGCTACCGTGGCGAATGGTCAGCGCCATCTGCTCAACAGTCATGGTTTCTGCCGGGAAGATCATATCACCTTCGTTTTCACGGTCTTCATCATCAAGCACCATTACACCGCGTCCTTCACGCAGCGCAGCCAGTGCATTTTCAACACGTTC  >  NC_000913/3184276‑3184427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑