Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,163	T→C	Y426H (TAT→CAT)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,163	0	T	C	100.0%	165.2 / NA	47	Y426H (TAT→CAT)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/29);  total (18/29)

GAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACGT  >  NC_000913/3230085‑3230240                                                                               |                                                                              gAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGcat                                                                             <  1:602535/81‑1 (MQ=255)     gtggtgGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACg                                                                         <  1:602536/81‑1 (MQ=255)        gtggCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGAGCATAACGTCa                                                                      <  2:602537/81‑1 (MQ=255)          ggCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCAcc                                                                    >  2:602601/1‑81 (MQ=255)           gCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCAcc                                                                    <  2:602538/80‑1 (MQ=255)           gCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCAcc                                                                    >  1:602616/1‑80 (MQ=255)             gATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCaa                                                                 <  1:602539/81‑1 (MQ=255)              aTGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAg                                                                <  2:602540/81‑1 (MQ=255)               tGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCAccc                                                                   >  1:602602/1‑77 (MQ=255)                ggcggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTTGGTGATGGAAGTGCATAACGTCACCCAAGa                                                               >  2:602611/1‑80 (MQ=255)                ggcggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGa                                                               >  2:602606/1‑80 (MQ=255)                 gcggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATa                                                             <  1:602541/81‑1 (MQ=255)                  cggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATaa                                                            <  1:602542/81‑1 (MQ=255)                      aaCTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAAcc                                                        >  1:602620/1‑81 (MQ=255)                       aCTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCt                                                       <  1:602543/81‑1 (MQ=255)                         tGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTgg                                                     >  2:602621/1‑81 (MQ=255)                            aacgaacgTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTg                                                  >  2:602623/1‑81 (MQ=255)                             acgaacgTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTgg                                                 <  2:602545/81‑1 (MQ=255)                             acgaacgTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTgg                                                 <  1:602544/81‑1 (MQ=255)                              cgaacgTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGc                                                <  1:602546/81‑1 (MQ=255)                                aacgTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCag                                              <  1:602547/81‑1 (MQ=255)                                      aTACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATg                                         <  1:602548/80‑1 (MQ=255)                                          cAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTAccc                                    <  1:602549/81‑1 (MQ=255)                                           aaaCCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTAccc                                    >  1:602613/1‑80 (MQ=255)                                            aaCCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGa                                  <  1:602550/81‑1 (MQ=255)                                                  gCCTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACtgg                            <  2:602551/81‑1 (MQ=255)                                                   ccTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACtggt                           <  2:602552/81‑1 (MQ=255)                                                    cTTGCCGCCTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACtggtg                          <  2:602553/81‑1 (MQ=255)                                                    cTTGCCGCCTGGTCGGTGATGGAAGTGCAT‑ACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACtggtgg                         >  2:602607/1‑81 (MQ=255)                                                       gccgccTGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGcc                       >  2:602632/1‑81 (MQ=255)                                                             tGGTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCAc                 >  2:602617/1‑81 (MQ=255)                                                               gTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCAc                 <  2:602555/79‑1 (MQ=255)                                                               gTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACg                <  1:602554/80‑1 (MQ=255)                                                               gTCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGa               >  2:602635/1‑81 (MQ=255)                                                                tCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGAc              <  2:602556/81‑1 (MQ=255)                                                                tCGGTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGAc              <  1:602557/81‑1 (MQ=255)                                                                  ggTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtg            >  1:602637/1‑81 (MQ=255)                                                                  ggTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtg            <  1:602559/81‑1 (MQ=255)                                                                  ggTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACt             <  2:602560/80‑1 (MQ=255)                                                                  ggTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGAGTGACAGAGATGTACCCGAAACTGGTGGCCTATCACGACt             <  2:602558/80‑1 (MQ=255)                                                                   gTGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtgg           <  2:602561/81‑1 (MQ=255)                                                                    tGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtggt          <  1:602562/81‑1 (MQ=255)                                                                    tGATGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtggt          >  2:602625/1‑81 (MQ=255)                                                                      aTGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtggtgg        <  2:602563/81‑1 (MQ=255)                                                                      aTGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtggtgg        >  2:602619/1‑81 (MQ=255)                                                                      aTGGAAGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACtggtg         <  2:602564/80‑1 (MQ=255)                                                                          aaGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTAc    >  1:602638/1‑81 (MQ=255)                                                                           aGTGCATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACg   >  1:602612/1‑81 (MQ=255)                                                                            gtgcATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACGt  <  2:602565/81‑1 (MQ=255)                                                                               |                                                                              GAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACGT  >  NC_000913/3230085‑3230240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑