Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,105	T→G	H110P (CAC→CCC)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,105	0	T	G	100.0%	75.4 / NA	23	H110P (CAC→CCC)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/12);  total (11/12)

ATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCG  >  NC_000913/334032‑334176                                                                          |                                                                        ataCACTTCTGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTggg                                                                  >  2:76075/1‑81 (MQ=255)    cacTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCg                                                                >  1:76071/1‑80 (MQ=255)     acTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGta                                                              >  1:76068/1‑81 (MQ=255)     acTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGta                                                              >  1:76074/1‑81 (MQ=255)           gtggtgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAagc                                                        <  1:76041/81‑1 (MQ=255)               tgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCt                                                    <  1:76042/81‑1 (MQ=255)               tgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCt                                                    <  1:76043/81‑1 (MQ=255)                gACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCt                                                    >  2:76063/1‑80 (MQ=255)                gACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCt                                                    >  2:229941/1‑80 (MQ=255)                    gTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTa                                               <  1:76044/81‑1 (MQ=255)                         cTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATAc                                           >  2:76091/1‑80 (MQ=255)                             cAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATg                                      >  1:76070/1‑81 (MQ=255)                                    gAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCacac                               <  2:76045/81‑1 (MQ=255)                                    gAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCacac                               <  2:76046/81‑1 (MQ=255)                                                 cAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGcc                  >  2:76086/1‑81 (MQ=255)                                                 cAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGc                   <  2:76047/80‑1 (MQ=255)                                                 cAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGc                   <  1:76048/80‑1 (MQ=255)                                                   gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                <  2:76049/81‑1 (MQ=255)                                                    cACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTg               <  2:76050/81‑1 (MQ=255)                                                    cACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTg               >  2:76067/1‑81 (MQ=255)                                                      ccTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGCTTAATACAAATACGCACACGCGCTTCTATGCCCTGc              >  2:76088/1‑80 (MQ=255)                                                              aaGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTa     <  1:445664/81‑1 (MQ=255)                                                                 cGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCg  <  2:76051/81‑1 (MQ=255)                                                                          |                                                                        ATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCG  >  NC_000913/334032‑334176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑