Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,047	A→T	Y90N (TAT→AAT)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,047	0	A	T	100.0%	155.1 / NA	43	Y90N (TAT→AAT)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/22);  total (21/22)

GTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242970‑3243123                                                                              |                                                                             gTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCt                                                                            >  1:605766/1‑80 (MQ=255)    aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                        >  1:605756/1‑81 (MQ=255)     gcgcGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATcc                                                                       <  1:605704/81‑1 (MQ=255)      cgcgACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                        >  1:605789/1‑79 (MQ=255)         gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                        >  1:605810/1‑76 (MQ=255)         gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGAt                                                                   <  1:605705/81‑1 (MQ=255)               aGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                               >  1:605760/1‑79 (MQ=255)                gATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                               >  1:605752/1‑78 (MQ=255)                gATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCa                                                             <  2:605706/80‑1 (MQ=255)                 aTCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                               >  1:605753/1‑77 (MQ=255)                      cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGt                                                      <  2:605708/81‑1 (MQ=255)                      cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGt                                                      <  2:605707/81‑1 (MQ=255)                        cTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTgc                                                    >  1:605762/1‑81 (MQ=255)                                  tGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtgtg                                          >  2:605770/1‑81 (MQ=255)                                    ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTAtt                                             <  1:605710/76‑1 (MQ=255)                                    ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtgt                                           <  2:605711/78‑1 (MQ=255)                                    ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTggg                                        >  2:605771/1‑81 (MQ=255)                                    ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTggg                                        <  2:605709/81‑1 (MQ=255)                                       cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt                                     <  1:605712/81‑1 (MQ=255)                                          gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtga                                   <  1:605713/80‑1 (MQ=255)                                           cgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaa                                  <  1:605715/80‑1 (MQ=255)                                           cgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaa                                  <  1:605714/80‑1 (MQ=255)                                             caggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               <  2:605716/81‑1 (MQ=255)                                                gcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGTcgtgaacgtgaa                            >  1:605780/1‑81 (MQ=255)                                                gcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtgaa                            <  1:605717/81‑1 (MQ=255)                                                 cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtgaa                            >  2:605768/1‑80 (MQ=255)                                                 cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCgtgaacgtgaac                           <  2:605718/81‑1 (MQ=255)                                                  agATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAaca                          >  2:605775/1‑81 (MQ=255)                                                   gATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAacac                         >  2:605783/1‑81 (MQ=255)                                                   gATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAacac                         <  1:605719/81‑1 (MQ=255)                                                        tGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc                    >  2:605800/1‑81 (MQ=255)                                                          aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg                   <  1:605720/80‑1 (MQ=255)                                                             aTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAat               <  1:605721/81‑1 (MQ=255)                                                              tCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAata              <  1:605723/81‑1 (MQ=255)                                                              tCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAata              <  1:605722/81‑1 (MQ=255)                                                                 ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           <  2:605724/81‑1 (MQ=255)                                                                  gTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCg          <  1:605725/81‑1 (MQ=255)                                                                   ttGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGc         >  2:605793/1‑81 (MQ=255)                                                                     gATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGcc        >  1:605781/1‑80 (MQ=255)                                                                     gATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGcc        >  2:605786/1‑80 (MQ=255)                                                                         gCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATg    >  2:605767/1‑80 (MQ=255)                                                                           gATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCa  >  1:605774/1‑80 (MQ=255)                                                                           gATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCa  >  1:605769/1‑80 (MQ=255)                                                                              |                                                                             GTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242970‑3243123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑