Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,401	G→T	H204N (CAT→AAT)	cyuP ←	putative D/L‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,401	0	G	T	100.0%	146.9 / NA	41	H204N (CAT→AAT)	cyuP	putative D/L‑serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/17);  total (24/17)

AGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTG  >  NC_000913/3257325‑3257477                                                                             |                                                                             aGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCca                                                                           >  2:609169/1‑80 (MQ=255)  gggTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCcaca                                                                         >  1:609160/1‑81 (MQ=255)   ggTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACAt                                                                        >  2:609167/1‑81 (MQ=255)    gTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACAtt                                                                       >  2:609157/1‑81 (MQ=255)    gTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACAtt                                                                       <  1:609122/81‑1 (MQ=255)          ggCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccacc                                                                 <  1:609123/81‑1 (MQ=255)           gCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccacca                                                                <  1:609124/81‑1 (MQ=255)                gTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGac                                                           <  1:609125/81‑1 (MQ=255)                  aataatGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGacac                                                         >  2:609166/1‑81 (MQ=255)                     aatGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGcc                                                      >  1:609170/1‑81 (MQ=255)                      atGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGcc                                                      <  2:609126/80‑1 (MQ=255)                        ggCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAg                                                   >  2:609150/1‑81 (MQ=255)                         gCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAgc                                                  <  1:609127/81‑1 (MQ=255)                            tttttCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGcgcc                                               <  2:609128/81‑1 (MQ=255)                            tttttCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGcgcc                                               <  2:609129/81‑1 (MQ=255)                               ttCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGcgccgc                                             <  2:609130/80‑1 (MQ=255)                                tCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCcgcg                                            <  1:609131/80‑1 (MQ=255)                                 cACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGac                                          >  1:609174/1‑81 (MQ=255)                                 cACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGac                                          >  2:609176/1‑81 (MQ=255)                                  aCCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGac                                          >  2:609172/1‑80 (MQ=255)                                     agcagcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCTCACCAGCGACACGCCCAGCGCCGCGaccac                                       >  2:609178/1‑80 (MQ=255)                                     agcagcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGaccac                                       >  2:609184/1‑80 (MQ=255)                                       cagcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag                                    >  1:609171/1‑81 (MQ=255)                                       cagcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag                                    <  1:609132/81‑1 (MQ=255)                                             cAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCtt                              >  1:609173/1‑81 (MQ=255)                                              aGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTg                             <  2:609133/81‑1 (MQ=255)                                                    ggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAg                        >  2:609182/1‑80 (MQ=255)                                                      tAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCAc                     >  1:609185/1‑81 (MQ=255)                                                         cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt                  <  2:609135/81‑1 (MQ=255)                                                         cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt                  <  1:609134/81‑1 (MQ=255)                                                              cGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCgg             >  2:609191/1‑81 (MQ=255)                                                              cGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCgg             <  1:609136/81‑1 (MQ=255)                                                              cGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCgg             >  1:609177/1‑81 (MQ=255)                                                               gACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGt            >  1:609188/1‑81 (MQ=255)                                                                aCGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc           <  1:609137/81‑1 (MQ=255)                                                                 cGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc           >  2:609168/1‑80 (MQ=255)                                                                    tataCAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaaa       >  1:609181/1‑81 (MQ=255)                                                                     ataCAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaaa       <  1:609138/80‑1 (MQ=255)                                                                       aCAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAttt    >  2:609179/1‑81 (MQ=255)                                                                        cAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtttt   >  2:609175/1‑81 (MQ=255)                                                                          gATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTg  >  2:609189/1‑80 (MQ=255)                                                                             |                                                                             AGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTG  >  NC_000913/3257325‑3257477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑