Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,264,112	C→G	A302A (GCG→GCC)	tdcC ←	threonine/serine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,264,112	0	C	G	100.0%	110.4 / NA	32	A302A (GCG→GCC)	tdcC	threonine/serine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/20);  total (12/20)

AGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGA  >  NC_000913/3264036‑3264183                                                                             |                                                                        aGATAGCCACGGGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCaa                                                                     <  1:610728/81‑1 (MQ=255)   aTAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAg                                                                   >  2:610761/1‑81 (MQ=255)      gCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTgg                                                                >  2:610776/1‑81 (MQ=255)         aCGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGt                                                               >  1:610779/1‑79 (MQ=255)           gAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGc                                                           <  1:610729/81‑1 (MQ=255)             gTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGc                                                           >  1:610785/1‑79 (MQ=255)              tGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAg                                                        <  1:610730/81‑1 (MQ=255)                cGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGAt                                                      <  1:610732/81‑1 (MQ=255)                cGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGAt                                                      <  2:610731/81‑1 (MQ=255)                  atgatgGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATaa                                                    <  1:610733/81‑1 (MQ=255)                      tgGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGaaa                                                <  1:610734/81‑1 (MQ=255)                      tgGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGa                                                  >  1:610791/1‑79 (MQ=255)                       ggAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAg                                               >  1:610787/1‑81 (MQ=255)                         aaGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCa                                             <  1:610735/81‑1 (MQ=255)                           gCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACt                                           >  2:610774/1‑81 (MQ=255)                             cGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTgg                                         <  2:610736/81‑1 (MQ=255)                             cGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTgg                                         <  1:610737/81‑1 (MQ=255)                                   ttCCAGTGTAATCGCGAACGTTGTTTTGGTATCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAatat                                    <  2:610738/80‑1 (MQ=255)                                     ccAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTc                                  <  2:610739/80‑1 (MQ=255)                                               cgcgAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGc                       >  1:610782/1‑81 (MQ=255)                                                gcgAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCt                      <  2:610740/81‑1 (MQ=255)                                                  gAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTc                    >  1:610781/1‑81 (MQ=255)                                                  gAACGTTGTTTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTc                    >  1:610777/1‑81 (MQ=255)                                                      gttgttTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGcc                <  2:610741/81‑1 (MQ=255)                                                         gttTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCAt              <  2:610743/80‑1 (MQ=255)                                                         gttTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCAt              <  1:610742/80‑1 (MQ=255)                                                         gttTTGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATg             >  2:610795/1‑81 (MQ=255)                                                          ttttGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATg             <  2:610744/80‑1 (MQ=255)                                                             tGGTACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTg          <  2:610745/80‑1 (MQ=255)                                                                tACCGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCg      <  2:610746/81‑1 (MQ=255)                                                                  ccGGTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGga    <  2:610747/81‑1 (MQ=255)                                                                    ggTCATGGAGGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGgaga  >  1:610786/1‑81 (MQ=255)                                                                             |                                                                        AGATAGCCACGAGTGCGATGATGGAAGCCGCATATTCCAGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGA  >  NC_000913/3264036‑3264183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑