Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,265,761	C→A	V90F (GTT→TTT)	tdcB ←	catabolic threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,265,761	0	C	A	100.0%	111.5 / NA	31	V90F (GTT→TTT)	tdcB	catabolic threonine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/13);  total (18/13)

CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT  >  NC_000913/3265684‑3265838                                                                              |                                                                              cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAaccc                                                                            >  2:611150/1‑81 (MQ=255)  gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt                                                                           >  1:611167/1‑81 (MQ=255)  gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt                                                                           >  1:611140/1‑81 (MQ=255)  gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt                                                                           >  1:611152/1‑81 (MQ=255)     aCTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTgc                                                                        >  1:611166/1‑81 (MQ=255)      cTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTg                                                                         <  2:611114/79‑1 (MQ=255)           gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg                                                                  >  2:611149/1‑81 (MQ=255)           gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg                                                                  <  2:611115/81‑1 (MQ=255)           gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg                                                                   <  1:611116/80‑1 (MQ=255)                tGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGcc                                                             >  2:611160/1‑81 (MQ=255)                        cTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAAc                                                     <  1:611117/81‑1 (MQ=255)                          tttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAg                                                   >  1:611171/1‑81 (MQ=255)                           ttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAgg                                                  >  2:611159/1‑81 (MQ=255)                              gCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGcc                                                <  1:611118/80‑1 (MQ=255)                               cATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac                                              <  1:611120/81‑1 (MQ=255)                               cATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac                                              <  2:611119/81‑1 (MQ=255)                                aTCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac                                              >  1:611157/1‑80 (MQ=255)                                aTCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac                                              >  2:611162/1‑80 (MQ=255)                                        tttACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCttt                                     >  1:611168/1‑81 (MQ=255)                                           aCCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGc                                   <  1:611121/80‑1 (MQ=255)                                            ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGt                                 >  1:611164/1‑81 (MQ=255)                                            ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGt                                 >  1:611173/1‑81 (MQ=255)                                                cGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGttttt                             >  2:611163/1‑81 (MQ=255)                                                             cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg                >  2:611165/1‑81 (MQ=255)                                                             cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg                >  1:611170/1‑81 (MQ=255)                                                              gcgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg                <  1:611123/80‑1 (MQ=255)                                                              gcgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg                <  1:611122/80‑1 (MQ=255)                                                                 cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa            <  2:611124/81‑1 (MQ=255)                                                                 cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa            <  1:611125/81‑1 (MQ=255)                                                                     agagGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACtt         >  2:611156/1‑80 (MQ=255)                                                                         ggAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAttt    <  2:611126/81‑1 (MQ=255)                                                                          gAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATtta   >  2:611180/1‑81 (MQ=255)                                                                           aaaaCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATttat  <  1:611127/81‑1 (MQ=255)                                                                              |                                                                              CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT  >  NC_000913/3265684‑3265838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑