Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,313,786	C→T	L743L (CTG→CTA)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,313,786	0	C	T	100.0%	181.1 / NA	50	L743L (CTG→CTA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/24);  total (26/24)

GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC  >  NC_000913/3313708‑3313858                                                                               |                                                                         ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                        >  1:622724/1‑81 (MQ=255) ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                        >  2:622717/1‑81 (MQ=255) ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg                                                                        >  2:622714/1‑81 (MQ=255)      agtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATgg                                                                   >  1:622721/1‑81 (MQ=255)       gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc                                                                  >  1:622737/1‑81 (MQ=255)       gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc                                                                  <  2:622653/81‑1 (MQ=255)       gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc                                                                  >  1:622711/1‑81 (MQ=255)        taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCg                                                                 <  1:622654/81‑1 (MQ=255)        taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCg                                                                 <  2:622655/81‑1 (MQ=255)          aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt                                                               <  2:622656/81‑1 (MQ=255)          aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt                                                               <  2:622657/81‑1 (MQ=255)          aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt                                                               <  2:622658/81‑1 (MQ=255)            gCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTg                                                              >  1:622718/1‑80 (MQ=255)              aGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAc                                                           <  1:622659/81‑1 (MQ=255)                 tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgcc                                                        <  2:622661/81‑1 (MQ=255)                 tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgcc                                                        <  1:622660/81‑1 (MQ=255)                      gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGc                                                   <  1:622662/81‑1 (MQ=255)                        tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGc                                                   >  1:622720/1‑79 (MQ=255)                             gCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTg                                            >  1:622751/1‑81 (MQ=255)                                tCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg                                         >  2:622750/1‑81 (MQ=255)                                tCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg                                         >  2:622719/1‑81 (MQ=255)                                 cAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg                                         <  2:622663/80‑1 (MQ=255)                                  aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTc                                       <  2:622664/81‑1 (MQ=255)                                      aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGtt                                   >  2:622731/1‑81 (MQ=255)                                        tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTc                                 >  2:622729/1‑81 (MQ=255)                                          tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCgg                               >  1:622749/1‑81 (MQ=255)                                               gCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATa                          >  2:622725/1‑81 (MQ=255)                                                cAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATac                         >  2:622741/1‑81 (MQ=255)                                                 agagGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacc                        <  1:622665/81‑1 (MQ=255)                                                  gagGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca                       >  1:622728/1‑81 (MQ=255)                                                  gagGCATCAGCACGAACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca                       >  2:622734/1‑81 (MQ=255)                                                   agGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                      >  1:622738/1‑81 (MQ=255)                                                    ggCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                     >  2:622736/1‑81 (MQ=255)                                                    ggCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                     <  2:622666/81‑1 (MQ=255)                                                     gCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt                    <  2:622667/81‑1 (MQ=255)                                                       aTCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc                  >  1:622747/1‑81 (MQ=255)                                                        tCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTAGGTGATACCACCTACg                 <  1:622668/81‑1 (MQ=255)                                                          aGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc                <  1:622669/80‑1 (MQ=255)                                                            cACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAg             <  1:622670/81‑1 (MQ=255)                                                                tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc         <  1:622671/81‑1 (MQ=255)                                                                 acgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc         >  1:622723/1‑80 (MQ=255)                                                                  cgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCg        <  1:622672/80‑1 (MQ=255)                                                                   gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat      >  1:622733/1‑81 (MQ=255)                                                                   gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat      >  1:622745/1‑81 (MQ=255)                                                                   gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat      >  1:622752/1‑81 (MQ=255)                                                                    ttAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg     <  2:622673/81‑1 (MQ=255)                                                                    ttAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg     <  2:622674/81‑1 (MQ=255)                                                                      aaaGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat   >  1:622732/1‑81 (MQ=255)                                                                       aaGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc  <  2:622675/81‑1 (MQ=255)                                                                       aaGCCAACTAGGATGGCGTTGGACGAAGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat   <  1:622676/80‑1 (MQ=255)                                                                               |                                                                         GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC  >  NC_000913/3313708‑3313858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑