Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,314,620	T→C	V465V (GTA→GTG)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,314,620	0	T	C	100.0%	159.6 / NA	46	V465V (GTA→GTG)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/26);  total (20/26)

TACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAG  >  NC_000913/3314542‑3314692                                                                               |                                                                         tACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCAc                                                                        <  1:622913/81‑1 (MQ=255)    cTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGAt                                                                     <  2:622914/81‑1 (MQ=255)       cgccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTc                                                                  <  1:622915/81‑1 (MQ=255)        gccgcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTcc                                                                 <  1:622916/81‑1 (MQ=255)           gcTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGtt                                                              >  1:622981/1‑81 (MQ=255)              ttCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGcc                                                           >  2:622973/1‑81 (MQ=255)                 gcgTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgc                                                        <  2:622917/81‑1 (MQ=255)                   gTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgcg                                                       >  2:622993/1‑80 (MQ=255)                   gTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTgcg                                                       >  1:622966/1‑80 (MQ=255)                    tGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGca                                                     >  1:622967/1‑81 (MQ=255)                     gCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcac                                                    >  1:622976/1‑81 (MQ=255)                         gATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcaccac                                                 <  1:622919/80‑1 (MQ=255)                         gATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcaccac                                                 <  2:622921/80‑1 (MQ=255)                         gATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcaccac                                                 <  2:622920/80‑1 (MQ=255)                         gATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGcaccac                                                 <  1:622918/80‑1 (MQ=255)                                 cGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGc                                         <  2:622922/80‑1 (MQ=255)                                  gATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCAt                                       >  2:622989/1‑81 (MQ=255)                                     ggTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTg                                     >  1:622983/1‑80 (MQ=255)                                       tCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAg                                  >  2:622978/1‑81 (MQ=255)                                          gCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTa                                >  1:622979/1‑80 (MQ=255)                                           cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGt                                 <  2:622923/78‑1 (MQ=255)                                           cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTaaa                              <  2:622924/81‑1 (MQ=255)                                           cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTaa                               <  2:622926/80‑1 (MQ=255)                                           cGGCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTa                                <  1:622925/79‑1 (MQ=255)                                            ggCATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAAc                             <  2:622927/81‑1 (MQ=255)                                              cATCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAAcgc                           >  2:622988/1‑81 (MQ=255)                                                tCACACCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACgcgg                         >  2:623000/1‑81 (MQ=255)                                                  acacCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACgcggcg                       >  1:623034/1‑81 (MQ=255)                                                  acacCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACgcggcg                       <  2:622928/81‑1 (MQ=255)                                                  acacCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACgcggc                        <  1:622929/80‑1 (MQ=255)                                                    acCGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTg                     <  1:622930/81‑1 (MQ=255)                                                     ccGTCGTCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGc                    <  1:622931/81‑1 (MQ=255)                                                      cgtcgtCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGcc                   <  2:622932/81‑1 (MQ=255)                                                      cgtcgtCGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGcc                   >  1:622984/1‑81 (MQ=255)                                                       gtcgtcGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGccc                  >  2:622977/1‑81 (MQ=255)                                                       gtcgtcGGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGccc                  <  1:622933/81‑1 (MQ=255)                                                          gtcgGCAGCAACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCgg               >  2:622975/1‑81 (MQ=255)                                                               cagcaACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGgtgt          >  1:622991/1‑81 (MQ=255)                                                               cagcaACAACCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGgtgt          >  1:622990/1‑81 (MQ=255)                                                                  caacaaCCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCa       <  2:622934/81‑1 (MQ=255)                                                                   aacaacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAg      >  1:622994/1‑81 (MQ=255)                                                                    acaacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAgg     <  2:622935/81‑1 (MQ=255)                                                                    acaacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAgg     <  1:622936/81‑1 (MQ=255)                                                                     caacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGa    <  1:622937/81‑1 (MQ=255)                                                                      aacCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGaa   <  2:622938/81‑1 (MQ=255)                                                                       acCAGGACCACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAg  >  2:622980/1‑81 (MQ=255)                                                                               |                                                                         TACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAG  >  NC_000913/3314542‑3314692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑