Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,315,439	A→G	R192R (CGT→CGC)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,315,439	0	A	G	100.0%	239.5 / NA	68	R192R (CGT→CGC)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/29);  total (39/29)

GTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTTTT  >  NC_000913/3315361‑3315515                                                                               |                                                                             gatATCAGTCCATTTGTTTTCTTCCGCCATACGACGCGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTacggcg                                                                            >  1:623271/3‑81 (MQ=255) gTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTacggcg                                                                            >  1:623268/1‑81 (MQ=255)  ttATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGgcgc                                                                           <  1:623170/81‑1 (MQ=255)  ttATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGgcgc                                                                           <  1:623169/81‑1 (MQ=255)     tCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGct                                                                        >  1:623244/1‑81 (MQ=255)       aGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGctt                                                                       <  1:623171/80‑1 (MQ=255)        gTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttct                                                                     <  2:623173/81‑1 (MQ=255)        gTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttct                                                                     >  2:623247/1‑81 (MQ=255)        gTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttct                                                                     <  1:623172/81‑1 (MQ=255)         tCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttctt                                                                    <  1:623174/81‑1 (MQ=255)           cATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttcttc                                                                   <  2:623175/80‑1 (MQ=255)            aTTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttcttctt                                                                 >  1:623232/1‑81 (MQ=255)             tttGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttcttctt                                                                 >  2:623235/1‑80 (MQ=255)             tttGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCttcttcttc                                                                <  1:623176/81‑1 (MQ=255)              ttGTTTTCTTCCGCCATGCGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGcttcttctt                                                                 >  2:623266/1‑79 (MQ=255)               tGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCa                                                               <  2:623177/80‑1 (MQ=255)               tGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAg                                                              >  2:623263/1‑81 (MQ=255)               tGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCCTCTTCGAGTTTACGGCGCGCTTCTTCTTCAg                                                              >  2:623264/1‑81 (MQ=255)                gTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGc                                                             >  2:623248/1‑81 (MQ=255)                  tttCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCtt                                                           >  1:623258/1‑81 (MQ=255)                   ttcttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCtt                                                           >  2:623243/1‑80 (MQ=255)                    tcttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCttt                                                          <  1:623178/80‑1 (MQ=255)                     cttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTAc                                                        >  1:623241/1‑81 (MQ=255)                      ttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACg                                                       >  1:623237/1‑81 (MQ=255)                       tcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACTGCGCGCTTCTTCTTCAGCTTTACGc                                                      >  1:623256/1‑81 (MQ=255)                         cGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCtt                                                    <  2:623180/81‑1 (MQ=255)                         cGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCtt                                                    <  1:623181/81‑1 (MQ=255)                         cGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCt                                                     <  1:623179/80‑1 (MQ=255)                          gCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTTAGCTTTACGCTTg                                                   <  2:623183/81‑1 (MQ=255)                          gCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTg                                                   <  1:623182/81‑1 (MQ=255)                             aTACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGc                                                <  2:623184/81‑1 (MQ=255)                                cgacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGctct                                             <  1:623185/81‑1 (MQ=255)                                 gacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTg                                            >  2:623234/1‑81 (MQ=255)                                 gacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTg                                            >  1:623217/1‑81 (MQ=255)                                 gacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTg                                            >  1:623272/1‑81 (MQ=255)                                 gacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTg                                            >  2:623236/1‑81 (MQ=255)                                   cgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTg                                            >  2:623242/1‑79 (MQ=255)                                    gTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAg                                         <  1:623186/81‑1 (MQ=255)                                     tGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGc                                        >  2:623261/1‑81 (MQ=255)                                      gCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCt                                       >  2:623280/1‑81 (MQ=255)                                       cttcttCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCtt                                      <  2:623187/81‑1 (MQ=255)                                         tcttcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTcc                                    >  2:623251/1‑81 (MQ=255)                                         tcttcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTcc                                    >  2:623249/1‑81 (MQ=255)                                             cAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCt                                   <  2:623188/78‑1 (MQ=255)                                             cAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCt                                >  2:623286/1‑81 (MQ=255)                                               gCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCa                              >  1:623259/1‑81 (MQ=255)                                               gCAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCa                              >  2:623279/1‑81 (MQ=255)                                                cAACGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCAc                             <  2:623189/81‑1 (MQ=255)                                                 aaCGCGACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCAcg                            >  2:623267/1‑81 (MQ=255)                                                     cgACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCAcggcgg                        >  2:623288/1‑81 (MQ=255)                                                      gACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCggg                       >  1:623276/1‑81 (MQ=255)                                                      gACGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCggg                       <  2:623190/81‑1 (MQ=255)                                                       aCGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGc                      <  1:623192/81‑1 (MQ=255)                                                       aCGTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGc                      <  1:623191/81‑1 (MQ=255)                                                         gTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCtt                    >  2:623281/1‑81 (MQ=255)                                                         gTGCTTCTTCTTCGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCtt                    >  1:623296/1‑81 (MQ=255)                                                             ttcttcttcGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCttttt                 >  2:623269/1‑80 (MQ=255)                                                               cttcttcGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAg              >  2:623270/1‑81 (MQ=255)                                                                  cttcGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGcc            <  2:623193/80‑1 (MQ=255)                                                                   ttcGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTg          >  1:623273/1‑81 (MQ=255)                                                                    tcGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTgg         >  2:623254/1‑81 (MQ=255)                                                                     cGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCt           <  1:623196/78‑1 (MQ=255)                                                                     cGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCt           <  2:623194/78‑1 (MQ=255)                                                                     cGAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTgg         <  1:623195/80‑1 (MQ=255)                                                                      gAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGc       <  2:623198/81‑1 (MQ=255)                                                                      gAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGc       >  2:623291/1‑81 (MQ=255)                                                                      gAGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGc       <  1:623197/81‑1 (MQ=255)                                                                       aGTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCg      >  2:623282/1‑81 (MQ=255)                                                                        gTTTACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGt     >  1:623295/1‑81 (MQ=255)                                                                         tttACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtt    <  2:623199/81‑1 (MQ=255)                                                                           tACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtttt  >  2:623287/1‑81 (MQ=255)                                                                           tACGGCGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtttt  >  2:623309/1‑81 (MQ=255)                                                                            acggcgCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtttt  <  1:623200/80‑1 (MQ=255)                                                                            acggcgCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtttt  >  1:623262/1‑80 (MQ=255)                                                                               |                                                                             GTTATCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTTTT  >  NC_000913/3315361‑3315515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑