Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	341,434	C→G	P104A (CCG→GCG)	yahJ →	putative deaminase with metallo‑dependent hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	341,434	0	C	G	100.0%	134.2 / NA	39	P104A (CCG→GCG)	yahJ	putative deaminase with metallo‑dependent hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/22);  total (17/22)

CGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAC  >  NC_000913/341357‑341511                                                                              |                                                                              cgcgcCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGgcgc                                                                            <  2:77667/81‑1 (MQ=255)      cAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTAt                                                                       >  1:77713/1‑81 (MQ=255)      cAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTAt                                                                       <  2:77668/81‑1 (MQ=255)        gACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGa                                                                     <  2:77669/81‑1 (MQ=255)           cGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGc                                                                  >  1:77723/1‑81 (MQ=255)              ggAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTgg                                                               >  2:77715/1‑81 (MQ=255)               gagaTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGc                                                              <  2:77670/81‑1 (MQ=255)                 gaTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCgg                                                            >  1:77720/1‑81 (MQ=255)                     cAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTaa                                                         <  2:77671/80‑1 (MQ=255)                       ggACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCt                                                      >  2:77722/1‑81 (MQ=255)                           ggCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtg                                                  <  1:77672/81‑1 (MQ=255)                             cAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtgct                                                <  1:77673/81‑1 (MQ=255)                                     tCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccacc                                         <  2:77674/80‑1 (MQ=255)                                       gCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCcaccac                                       <  2:77675/80‑1 (MQ=255)                                       gCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCcaccac                                       <  2:77676/80‑1 (MQ=255)                                        cccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCAccc                                     >  2:77717/1‑81 (MQ=255)                                         ccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCcg                                    <  1:77677/81‑1 (MQ=255)                                          cTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCcgc                                   >  1:77728/1‑81 (MQ=255)                                            gcgcGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGa                                 >  2:77731/1‑81 (MQ=255)                                             cgcgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGAc                                <  2:77678/81‑1 (MQ=255)                                              gcgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACa                               >  2:77734/1‑81 (MQ=255)                                                gagaACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATg                             >  1:77719/1‑81 (MQ=255)                                                 agaACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATg                             >  2:77724/1‑80 (MQ=255)                                                   aaCAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCat                          <  2:77679/81‑1 (MQ=255)                                                    aCAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCata                         <  2:77680/81‑1 (MQ=255)                                                           gCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAtct                  >  1:77753/1‑81 (MQ=255)                                                            cATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAtctc                 <  2:77681/81‑1 (MQ=255)                                                            cATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAtc                   <  2:77682/79‑1 (MQ=255)                                                             aTCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCg                <  2:77683/81‑1 (MQ=255)                                                                  gACGCCACGCTGGCGGACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaaa           <  2:77684/81‑1 (MQ=255)                                                                  gACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaaa           <  2:77685/81‑1 (MQ=255)                                                                  gACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaa            >  2:77711/1‑80 (MQ=255)                                                                   aCGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaaaa          >  1:77737/1‑81 (MQ=255)                                                                    cGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAAc         >  1:77742/1‑81 (MQ=255)                                                                         cGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCtttt    <  1:77686/81‑1 (MQ=255)                                                                         cGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCttt     >  2:77730/1‑80 (MQ=255)                                                                           cTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAc  <  2:77687/81‑1 (MQ=255)                                                                           cTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAc  <  1:77688/81‑1 (MQ=255)                                                                           cTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAc  <  1:77689/81‑1 (MQ=255)                                                                           cTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAc  >  2:77725/1‑81 (MQ=255)                                                                              |                                                                              CGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTAC  >  NC_000913/341357‑341511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑