Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,328,268	A→G	F150L (TTT→CTT)	greA ←	transcription elongation factor GreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,328,268	0	A	G	100.0%	75.9 / NA	23	F150L (TTT→CTT)	greA	transcription elongation factor GreA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGC  >  NC_000913/3328192‑3328344                                                                             |                                                                             ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc                                                                           >  1:626435/1‑80 (MQ=255) ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc                                                                           >  1:626452/1‑80 (MQ=255) ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc                                                                           >  2:626446/1‑80 (MQ=255)      ctttctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACtt                                                                      <  1:626421/80‑1 (MQ=255)             tACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc                                                              >  2:626474/1‑81 (MQ=255)                  tACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGt                                                         <  1:626422/81‑1 (MQ=255)                     aTCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtttt                                                      >  1:626457/1‑81 (MQ=255)                                 aGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCacaac                                          <  1:626423/81‑1 (MQ=255)                                    aTTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcat                                        >  2:626453/1‑80 (MQ=255)                                    aTTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcat                                        >  2:626451/1‑80 (MQ=255)                                      tGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTTATGACCACAAcatcat                                     >  2:626448/1‑81 (MQ=255)                                                      gTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt                     <  1:626424/81‑1 (MQ=255)                                                       tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc                    <  2:626425/81‑1 (MQ=255)                                                        aTTCCACCTTAATTGCTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt                     >  2:626454/1‑79 (MQ=255)                                                          tCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAgg                 >  2:626477/1‑81 (MQ=255)                                                             aCCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCa              >  1:626455/1‑81 (MQ=255)                                                              ccTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCAc             <  2:626426/81‑1 (MQ=255)                                                                  aaTTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGa           <  1:626427/79‑1 (MQ=255)                                                                   aTTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCa        <  2:626428/81‑1 (MQ=255)                                                                       cTTCAAGTTCCACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAg    >  1:626465/1‑81 (MQ=255)                                                                       cTTCAAGTTCCACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAg    >  1:626463/1‑81 (MQ=255)                                                                          cAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGc  <  2:626429/80‑1 (MQ=255)                                                                          cAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGc  <  2:626430/80‑1 (MQ=255)                                                                             |                                                                             TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGC  >  NC_000913/3328192‑3328344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑