Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,963	C→A	A230E (GCG→GAG)	yrbG →	putative transport protein YrbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,963	0	C	A	100.0%	129.5 / NA	37	A230E (GCG→GAG)	yrbG	putative transport protein YrbG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/23);  total (14/23)

CCATCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGCGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAATATTGTCATCG  >  NC_000913/3340888‑3341037                                                                            |                                                                           ccATCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAggggg                                                                       >  2:629623/1‑81 (MQ=255)  cATCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGt                                                                      >  2:629619/1‑81 (MQ=255)   aTCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGtt                                                                     >  2:629620/1‑81 (MQ=255)    tCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTc                                                                    <  1:629565/81‑1 (MQ=255)      aGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCg                                                                   <  2:629566/80‑1 (MQ=255)        cGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCaa                                                                <  1:629567/81‑1 (MQ=255)        cGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCa                                                                 >  1:629618/1‑80 (MQ=255)            ttGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGt                                                            <  1:629568/81‑1 (MQ=255)              gACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTg                                                           >  2:629628/1‑80 (MQ=255)              gACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTg                                                           <  2:629569/80‑1 (MQ=255)               aCGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGaa                                                         <  1:629571/81‑1 (MQ=255)               aCGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGaa                                                         <  1:629570/81‑1 (MQ=255)                 gATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGaaaa                                                       >  2:629616/1‑81 (MQ=255)                   tGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACg                                                     <  1:629572/81‑1 (MQ=255)                     ggTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGa                                                    <  2:629574/80‑1 (MQ=255)                     ggTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGAc                                                   <  2:629575/81‑1 (MQ=255)                     ggTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGAc                                                   <  2:629573/81‑1 (MQ=255)                           aCGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACAtt                                                <  2:629576/78‑1 (MQ=255)                                aaTTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCgg                                        <  1:629577/81‑1 (MQ=255)                                 aTTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCgg                                        >  1:629631/1‑80 (MQ=255)                                     cTATAGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAata                                   <  1:629578/81‑1 (MQ=255)                                        tCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAatat                                  <  1:629579/79‑1 (MQ=255)                                             aCCAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGgc                           >  1:629629/1‑81 (MQ=255)                                              ccAGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGgcg                          <  1:629580/81‑1 (MQ=255)                                                aGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGgcgc                         >  1:629622/1‑80 (MQ=255)                                                aGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGgcgc                         >  2:629636/1‑80 (MQ=255)                                                aGCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGgcgc                         >  1:629630/1‑80 (MQ=255)                                                 gCCTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCaa                       <  1:629581/81‑1 (MQ=255)                                                   cTGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAAc                     <  2:629582/81‑1 (MQ=255)                                                    tGCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATAACATTGGCGCAAACa                    >  2:629625/1‑81 (MQ=255)                                                     gCCGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACAt                   >  2:629627/1‑81 (MQ=255)                                                       cGGAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACAttt                 <  1:629583/81‑1 (MQ=255)                                                         gAACTGGCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACAttttt               <  1:629584/81‑1 (MQ=255)                                                              ggCAACCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAatat          >  2:629639/1‑81 (MQ=255)                                                               gCAACCGCAATAGAGGGGTTTCGCAAA‑GTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAATATTg        <  1:629585/81‑1 (MQ=255)                                                                  aCCGCAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAATATTGTc      <  2:629586/81‑1 (MQ=255)                                                                      cAATAGAGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAATATTGTCATCg  <  2:629587/81‑1 (MQ=255)                                                                            |                                                                           CCATCAGCGAGTTGACGATGGGTCTGACGGCAATTGCTATCGGAACCAGCCTGCCGGAACTGGCAACCGCAATAGCGGGGGTTCGCAAAGGTGAAAACGACATTGCTGTCGGAAATATCATTGGCGCAAACATTTTTAATATTGTCATCG  >  NC_000913/3340888‑3341037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑