Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,371,572	C→T	G335S (GGT→AGT)	nanT ←	N‑acetylneuraminate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,371,572	0	C	T	100.0%	119.9 / NA	35	G335S (GGT→AGT)	nanT	N‑acetylneuraminate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/18);  total (17/18)

CAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGG  >  NC_000913/3371497‑3371647                                                                            |                                                                            cAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAgg                                                                         >  1:637364/1‑80 (MQ=255)  aaaTACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAgg                                                                         >  1:637379/1‑79 (MQ=255)        cGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCAcc                                                                 <  2:637289/81‑1 (MQ=255)         ggAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCAcc                                                                 >  2:637355/1‑80 (MQ=255)           aataatCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTAc                                                              <  1:637291/81‑1 (MQ=255)           aataatCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTAc                                                              <  2:637290/81‑1 (MQ=255)               atCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACgcag                                                          >  2:637363/1‑81 (MQ=255)               atCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACgcag                                                          >  2:637347/1‑81 (MQ=255)                 cagcagCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACgcagca                                                        >  1:637367/1‑81 (MQ=255)                     agcTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATcc                                                     >  2:637377/1‑80 (MQ=255)                     agcTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATcc                                                     >  1:637362/1‑80 (MQ=255)                           gAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCGGCATCCCAccgcc                                              >  1:637356/1‑81 (MQ=255)                           gAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccgcc                                              <  1:637292/81‑1 (MQ=255)                           gAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccgcc                                              >  1:637370/1‑81 (MQ=255)                           gAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccgcc                                              <  1:637293/81‑1 (MQ=255)                            aGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCAccgccg                                             >  2:637366/1‑81 (MQ=255)                              gCCAGCAGGCTACAAACGTACGCTTTCCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCcgcg                                           <  1:637294/81‑1 (MQ=255)                               ccAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCgcgc                                          <  1:637295/81‑1 (MQ=255)                                  gcagGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCaa                                       <  2:637296/81‑1 (MQ=255)                                  gcagGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCaa                                       <  1:637297/81‑1 (MQ=255)                                  gcagGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCa                                        >  2:637394/1‑80 (MQ=255)                                     ggCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGc                                    <  1:637298/81‑1 (MQ=255)                                          cAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCAc                                 <  1:637299/79‑1 (MQ=255)                                           aaaCGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTa                               >  2:637372/1‑80 (MQ=255)                                             aCGTACGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAg                            <  2:637300/81‑1 (MQ=255)                                                  cGCTTTGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAg                       <  2:637301/81‑1 (MQ=255)                                                   gCTTTGCGGGTTCTCAGCCAGTGACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGACCAg                       >  2:637376/1‑80 (MQ=255)                                                       tGCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGcac                    <  1:637302/79‑1 (MQ=255)                                                        gCGGGTTCCCAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACAt                  <  2:637303/80‑1 (MQ=255)                                                               cccAGCCAGTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTAc           <  1:637304/80‑1 (MQ=255)                                                                ccagccagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAg         >  1:637374/1‑81 (MQ=255)                                                                  agccagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTa       >  2:637385/1‑81 (MQ=255)                                                                     cagTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGc    <  2:637305/81‑1 (MQ=255)                                                                      agTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCg   >  2:637371/1‑81 (MQ=255)                                                                       gTCACTGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCgg  <  1:637306/81‑1 (MQ=255)                                                                            |                                                                            CAAATACCGGAATAATCAGCAGCTGCGAGGCCAGCAGGCTACAAACGTACGCTTTGCGGGTTCCCAGCCAGTCACCGAGGAAGCCACCTACGCAGCATCCCACCGCCGCGCCAAAGCCACTAAAGAACAGCACATTGGCTACAGTATGCGG  >  NC_000913/3371497‑3371647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑