Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,973	G→A	G369S (GGC→AGC)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,973	0	G	A	100.0%	117.1 / NA	33	G369S (GGC→AGC)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/13);  total (20/13)

GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATG  >  NC_000913/3375897‑3376039                                                                             |                                                                   ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGctc                                                                >  1:638380/1‑81 (MQ=255)   aGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTg                                                              <  2:638305/81‑1 (MQ=255)      aTTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGc                                                            >  1:638378/1‑80 (MQ=255)                 ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGttt                                                <  2:638307/81‑1 (MQ=255)                 ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGtt                                                 <  1:638306/80‑1 (MQ=255)                    ggTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATg                                             >  1:638353/1‑81 (MQ=255)                      tATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGt                                            >  2:638389/1‑80 (MQ=255)                       aTTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCa                                          >  2:638367/1‑81 (MQ=255)                        ttGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCAt                                         <  1:638308/81‑1 (MQ=255)                             gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGTCGTTTATGTCATTTGc                                     >  1:638362/1‑80 (MQ=255)                             gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGc                                     >  1:638373/1‑80 (MQ=255)                             gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGc                                     >  1:638360/1‑80 (MQ=255)                               gatgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAg                                  >  2:638387/1‑81 (MQ=255)                                 tgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTc                                <  1:638309/81‑1 (MQ=255)                                   attattATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCtt                              <  2:638310/81‑1 (MQ=255)                                    ttattaTGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTa                             <  1:638311/81‑1 (MQ=255)                                      attatGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTAt                            <  1:638312/80‑1 (MQ=255)                                         atGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTcc                         <  2:638313/80‑1 (MQ=255)                                           ggCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa                      >  2:638359/1‑81 (MQ=255)                                            gcgcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa                      >  1:638375/1‑80 (MQ=255)                                            gcgcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa                      <  2:638314/80‑1 (MQ=255)                                              gcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAatat                   >  2:638382/1‑81 (MQ=255)                                              gcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAatat                   >  1:638368/1‑81 (MQ=255)                                                 ggTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg                 >  1:638371/1‑80 (MQ=255)                                                  gTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg                 >  1:638365/1‑79 (MQ=255)                                                  gTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGc                <  1:638315/80‑1 (MQ=255)                                                   tCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAAAATCGCAg              <  2:638316/81‑1 (MQ=255)                                                    cATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc             >  1:638391/1‑81 (MQ=255)                                                       tGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCg           >  2:638390/1‑80 (MQ=255)                                                          cATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACt       <  2:638317/81‑1 (MQ=255)                                                            tttGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTa      >  2:638377/1‑80 (MQ=255)                                                               gtgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATg  >  1:638383/1‑81 (MQ=255)                                                                tgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATg  >  2:638386/1‑80 (MQ=255)                                                                             |                                                                   GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATG  >  NC_000913/3375897‑3376039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑