Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,209	G→A	L230L (CTG→CTA)	aaeR →	LysR‑type transcriptional regulator AaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,209	0	G	A	100.0%	112.0 / NA	32	L230L (CTG→CTA)	aaeR	LysR‑type transcriptional regulator AaeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

CGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCC  >  NC_000913/3390133‑3390285                                                                             |                                                                             cGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcgg                                                                          <  2:641666/81‑1 (MQ=255)  ggAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcggc                                                                         >  1:641714/1‑81 (MQ=255)    aaGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAAcggcgg                                                                       >  2:641704/1‑81 (MQ=255)       ggATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTg                                                                    <  1:641667/81‑1 (MQ=255)              gACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCg                                                                 <  1:641668/77‑1 (MQ=255)              gACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGAt                                                             >  1:641709/1‑81 (MQ=255)               aCTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATc                                                            <  1:641670/81‑1 (MQ=255)               aCTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATc                                                            <  1:641669/81‑1 (MQ=255)                     cTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTAc                                                      >  2:641720/1‑81 (MQ=255)                        aTCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTTCGCTGGCTAACGGCGGGTTCCGGGATCTCCTTCGtt                                                   >  2:641725/1‑80 (MQ=255)                        aTCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGt                                                    <  2:641671/80‑1 (MQ=255)                           ccACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGcc                                                 <  1:641672/80‑1 (MQ=255)                              caAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTg                                             <  1:641673/81‑1 (MQ=255)                                aGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAt                                           >  2:641718/1‑81 (MQ=255)                                  gAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAtgt                                         <  2:641674/81‑1 (MQ=255)                                   aaGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCCGAtgtg                                        >  2:641715/1‑81 (MQ=255)                                           tgACTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATc                                 >  1:641716/1‑80 (MQ=255)                                              cTAATGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACg                             <  1:641675/81‑1 (MQ=255)                                                aaTGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAAc                              >  1:641708/1‑78 (MQ=255)                                                 aTGATCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACga                            <  2:641676/79‑1 (MQ=255)                                                    aTCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCa                       <  2:641677/81‑1 (MQ=255)                                                     tCCGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCa                       >  2:641711/1‑80 (MQ=255)                                                      ccGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAAt                     <  2:641678/81‑1 (MQ=255)                                                      ccGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAAt                     >  1:641719/1‑81 (MQ=255)                                                       cGATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAAt                     <  2:641679/80‑1 (MQ=255)                                                        gATGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCg                   >  1:641721/1‑81 (MQ=255)                                                         aTGACGCTGGTGCGCTGGCTAACGGCGGGTGCCGGGAACGCCTACGTGCCGCGGAAGTGGGTGAGCAACGAGATCAAACGt                  >  1:641730/1‑81 (MQ=255)                                                              gCTGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTggg               >  2:641743/1‑79 (MQ=255)                                                                tGGTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCCGATGTGGGTGATCAACGAGATCAATCGTGGGGAGc           >  2:641722/1‑81 (MQ=255)                                                                 ggTGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCt          >  2:641717/1‑81 (MQ=255)                                                                   tGCGCTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTgg        <  1:641680/81‑1 (MQ=255)                                                                    gcgcTGGCTAACGGCGGGTGCCGGGATCGCCTACGTGCCGCCGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTg         >  2:641732/1‑79 (MQ=255)                                                                      gctggctaACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGAGGGTGATCAACGAGATCAATCGTGGGGAGCTg         >  1:641713/1‑77 (MQ=255)                                                                       ctggctaACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGAt    <  1:641681/81‑1 (MQ=255)                                                                         ggctaACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATcc  >  1:641724/1‑81 (MQ=255)                                                                          gctaACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATcc  <  1:641682/80‑1 (MQ=255)                                                                             |                                                                             CGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCC  >  NC_000913/3390133‑3390285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑