Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,408,537	T→C	L311L (TTA→CTA)	panF →	pantothenate:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,408,537	0	T	C	100.0%	149.8 / NA	43	L311L (TTA→CTA)	panF	pantothenate:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)

GTATGCACCTGGCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGTTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTCGACAATT  >  NC_000913/3408463‑3408614                                                                           |                                                                              gTATGCACCTGGCCGGAGCGTTGGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATgg                                                                         >  2:646465/1‑81 (MQ=255)   aTGCACCTGGCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGt                                                                        >  2:646485/1‑80 (MQ=255)        ccTGGCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGt                                                                  <  1:646407/81‑1 (MQ=255)           ggCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGtgct                                                               >  2:646474/1‑81 (MQ=255)            gCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGtgctg                                                              >  2:646480/1‑81 (MQ=255)              cGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGtgctgc                                                             >  2:646478/1‑80 (MQ=255)              cGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGtgctgc                                                             >  1:646486/1‑80 (MQ=255)               ggAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCa                                                           <  1:646408/81‑1 (MQ=255)                gAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGGTCCCAACGCTAATGGTAAAAGTGCTGCCAc                                                          <  1:646409/81‑1 (MQ=255)                gAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCAc                                                          >  1:646468/1‑81 (MQ=255)                    gTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGtt                                                      <  1:646410/81‑1 (MQ=255)                    gTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGtt                                                      >  1:646470/1‑81 (MQ=255)                     ttAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGttt                                                     <  1:646411/81‑1 (MQ=255)                      tAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTtg                                                    >  1:646463/1‑81 (MQ=255)                        ggTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTtgc                                                   <  2:646412/80‑1 (MQ=255)                            gggCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCg                                              <  2:646413/81‑1 (MQ=255)                              gCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCggg                                            <  2:646414/81‑1 (MQ=255)                                 gTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATc                                         >  1:646481/1‑81 (MQ=255)                                  tGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCt                                        >  2:646491/1‑81 (MQ=255)                                    aTCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCtt                                       <  1:646416/80‑1 (MQ=255)                                    aTCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCtt                                       <  2:646415/80‑1 (MQ=255)                                     tCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTcc                                     >  1:646487/1‑81 (MQ=255)                                     tCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTcc                                     <  1:646417/81‑1 (MQ=255)                                       cccGATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTg                                   <  2:646418/81‑1 (MQ=255)                                          gATCTCACCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCt                                >  1:646473/1‑81 (MQ=255)                                                aCCGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCAc                            <  2:646419/79‑1 (MQ=255)                                                 ccGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGa                         <  1:646420/81‑1 (MQ=255)                                                  cGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGAt                        <  2:646421/81‑1 (MQ=255)                                                  cGTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGAt                        <  2:646422/81‑1 (MQ=255)                                                   gTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATg                       <  2:646423/81‑1 (MQ=255)                                                   gTACCGGACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATg                       <  1:646424/81‑1 (MQ=255)                                                        ggACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGc                  <  1:646425/81‑1 (MQ=255)                                                        ggACCTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGc                  >  1:646471/1‑81 (MQ=255)                                                            cTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGAt               >  2:646479/1‑80 (MQ=255)                                                            cTGGTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATc              <  1:646426/81‑1 (MQ=255)                                                              ggTGATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCAt            >  2:646427/1‑81 (MQ=255)                                                                 gATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTc         >  1:646483/1‑81 (MQ=255)                                                                 gATCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCCTCCTGGCTGCACCGATGGCTGCGATCATGTc         >  2:646496/1‑81 (MQ=255)                                                                  aTCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTc         <  2:646428/80‑1 (MQ=255)                                                                  aTCCCAACGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTc         <  1:646429/80‑1 (MQ=255)                                                                       aaCGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTCGACAAt   >  2:646492/1‑81 (MQ=255)                                                                        aCGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTCGACAAtt  <  2:646430/81‑1 (MQ=255)                                                                        aCGCTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTCGACAAtt  <  2:646431/81‑1 (MQ=255)                                                                           |                                                                              GTATGCACCTGGCCGGAGCGTTAGGTCGGGCGGTGATCCCCGATCTCACCGTACCGGACCTGGTGATCCCAACGTTAATGGTAAAAGTGCTGCCACCGTTTGCTGCCGGGATCTTCCTGGCTGCACCGATGGCTGCGATCATGTCGACAATT  >  NC_000913/3408463‑3408614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑