Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,849	T→C	F66S (TTT→TCT)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,849	0	T	C	100.0%	94.7 / NA	28	F66S (TTT→TCT)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

CCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGC  >  NC_000913/3411772‑3411926                                                                              |                                                                              cccACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGa                                                                            <  2:647259/81‑1 (MQ=255)  ccaccaTATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTg                                                                             >  2:647294/1‑79 (MQ=255)      caTATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGa                                                                       >  2:647313/1‑81 (MQ=255)            aCATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTg                                                                    >  1:647308/1‑78 (MQ=255)              aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag                                                               <  1:647260/81‑1 (MQ=255)              aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag                                                               >  2:647309/1‑81 (MQ=255)                     aaaaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCAc                                                         <  2:647261/80‑1 (MQ=255)                       aaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACcgc                                                      <  1:647262/81‑1 (MQ=255)                              aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg                                                >  2:647299/1‑80 (MQ=255)                              aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg                                                <  2:647263/80‑1 (MQ=255)                                 gTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGaaaa                                            <  1:647264/81‑1 (MQ=255)                                         gAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                     >  1:647306/1‑80 (MQ=255)                                         gAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                    <  1:647265/81‑1 (MQ=255)                                            gCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgcagc                                 <  2:647266/81‑1 (MQ=255)                                            gCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgcag                                  >  2:647298/1‑80 (MQ=255)                                              cTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCAt                               <  2:647267/81‑1 (MQ=255)                                                     gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcatc                        <  2:647268/81‑1 (MQ=255)                                                     gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcat                         >  1:647303/1‑80 (MQ=255)                                                     gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcat                         >  2:647296/1‑80 (MQ=255)                                                        gaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCGGGGCAGCATGTACATCATg                     <  2:647269/81‑1 (MQ=255)                                                             gTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAg                >  2:647304/1‑81 (MQ=255)                                                              tttATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGa                    <  2:647270/76‑1 (MQ=255)                                                                   cGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACgg           >  1:647307/1‑80 (MQ=255)                                                                     aCTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaaa        <  1:647271/81‑1 (MQ=255)                                                                     aCTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaa         >  1:647295/1‑80 (MQ=255)                                                                      cTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaaaa       <  2:647272/81‑1 (MQ=255)                                                                          cTGTCTGAAATTATTGCAGAGTGCCACCTCATTCTTACAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg   <  1:1039640/81‑1 (MQ=255)                                                                           tGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc  >  2:647302/1‑81 (MQ=255)                                                                              |                                                                              CCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGC  >  NC_000913/3411772‑3411926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑