Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,553	G→A	A137V (GCC→GTC)	yrdD ←	putative DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,553	0	G	A	100.0%	102.8 / NA	29	A137V (GCC→GTC)	yrdD	putative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

CGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCC  >  NC_000913/3431480‑3431630                                                                          |                                                                              cgcgGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAAt                                                                          <  1:651797/79‑1 (MQ=255)    ggTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGaca                                                                     <  1:651798/81‑1 (MQ=255)        ttcttttcGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTcc                                                                 >  2:651831/1‑81 (MQ=255)               cGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGc                                                          >  2:651843/1‑81 (MQ=255)               cGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGc                                                          >  1:651849/1‑81 (MQ=255)                    aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATca                                                     >  2:651862/1‑81 (MQ=255)                    aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATca                                                     <  2:651800/81‑1 (MQ=255)                    aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATc                                                      <  2:651801/80‑1 (MQ=255)                    aGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATc                                                      <  2:651799/80‑1 (MQ=255)                     gTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATcac                                                    <  1:651802/81‑1 (MQ=255)                         cGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACaa                                                  >  2:651837/1‑79 (MQ=255)                              aaTGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGa                                            >  1:651852/1‑80 (MQ=255)                                 gACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATg                                        >  2:651834/1‑81 (MQ=255)                                 gACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATg                                        >  2:651855/1‑81 (MQ=255)                                  acacTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGt                                       <  1:651803/81‑1 (MQ=255)                                   cacTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGtt                                      <  1:651804/81‑1 (MQ=255)                                     cTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGtttt                                    <  1:651805/81‑1 (MQ=255)                                        aGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGcc                                 <  2:651806/81‑1 (MQ=255)                                           gCATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCAt                               >  1:651853/1‑80 (MQ=255)                                            cATTCTCCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATaa                             <  1:651807/81‑1 (MQ=255)                                                ctcCAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACgg                          >  1:651840/1‑80 (MQ=255)                                                  ccAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAg                       >  1:651836/1‑81 (MQ=255)                                                  ccAGCTATGGGTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAg                       >  2:651844/1‑81 (MQ=255)                                                    aGCTATGGGTTTGAAGTTAATGACAAATTGACAGTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAgcg                     >  1:651846/1‑81 (MQ=255)                                                            gTTTGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGATCGGCGCTGGa             >  1:651851/1‑81 (MQ=255)                                                             tttGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGAc            <  1:651808/81‑1 (MQ=255)                                                              ttGAAGTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGAc            >  2:651864/1‑80 (MQ=255)                                                                   gTTAATGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATg      <  2:651809/81‑1 (MQ=255)                                                                       aTGACAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGccc  <  1:651810/81‑1 (MQ=255)                                                                          |                                                                              CGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCC  >  NC_000913/3431480‑3431630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑