Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,803	A→G	C17C (TGT→TGC)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,803	0	A	G	100.0%	88.4 / NA	26	C17C (TGT→TGC)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

GAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTG  >  NC_000913/3469731‑3469879                                                                         |                                                                             gAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAg                                                                      >  1:661285/1‑81 (MQ=255)    aaTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGccc                                                                   >  1:661287/1‑81 (MQ=255)     aTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGcccc                                                                  <  2:661250/81‑1 (MQ=255)       aaCCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCa                                                                 >  1:661281/1‑80 (MQ=255)        aCCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCAt                                                                <  2:661251/80‑1 (MQ=255)         ccTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATAc                                                              <  1:661252/81‑1 (MQ=255)          cTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATAcc                                                             <  1:661253/81‑1 (MQ=255)             atatTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCa                                                            <  2:661254/79‑1 (MQ=255)              tatttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAAt                                                          >  2:661288/1‑80 (MQ=255)                tttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCa                                                        <  1:661255/80‑1 (MQ=255)                  tattaCCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATa                                                      <  1:661256/80‑1 (MQ=255)                    ttaCCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGa                                                    <  2:661258/80‑1 (MQ=255)                    ttaCCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGAt                                                   <  1:661257/81‑1 (MQ=255)                       ccaccTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGAttt                                                 <  1:661259/80‑1 (MQ=255)                       ccaccTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTa                                                <  1:661260/81‑1 (MQ=255)                         accTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAg                                               >  2:661282/1‑80 (MQ=255)                          ccTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGt                                              >  2:661284/1‑80 (MQ=255)                           cTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGTCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTa                                             >  1:661283/1‑80 (MQ=255)                                         cATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAAttt                               >  2:661279/1‑80 (MQ=255)                                                  aTTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATc                     <  2:661261/81‑1 (MQ=255)                                                  aTTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATc                     >  2:661280/1‑81 (MQ=255)                                                     gCCAGTGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCt                  >  1:661286/1‑81 (MQ=255)                                                       cAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTtg                >  1:661291/1‑81 (MQ=255)                                                        aaTGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTtgt               >  2:661292/1‑81 (MQ=255)                                                                     cGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTg  <  1:661262/81‑1 (MQ=255)                                                                      ggAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTg  >  1:661295/1‑80 (MQ=255)                                                                         |                                                                             GAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTG  >  NC_000913/3469731‑3469879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑