Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,702	A→T	F605I (TTT→ATT)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,702	0	A	T	100.0%	99.9 / NA	29	F605I (TTT→ATT)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/13);  total (16/13)

GTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCG  >  NC_000913/3471625‑3471775                                                                              |                                                                          gTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGcc                                                                        >  2:661746/1‑81 (MQ=255)  tCACCGGTGTTCTCTTCCGGAGTCTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCct                                                                       >  2:661749/1‑81 (MQ=255)     ccGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCcttct                                                                    <  2:661711/81‑1 (MQ=255)       ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCttt                                                                  <  2:661712/81‑1 (MQ=255)        gtgtTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTa                                                                 <  1:661713/81‑1 (MQ=255)                   cGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGa                                                      <  1:661714/81‑1 (MQ=255)                     gAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAag                                                    <  1:661715/81‑1 (MQ=255)                        tttCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAagcag                                                 <  1:661716/81‑1 (MQ=255)                           cTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAagcagc                                                >  2:661768/1‑79 (MQ=255)                              cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCa                                              <  2:661717/78‑1 (MQ=255)                               ttCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGtt                                           >  2:661750/1‑80 (MQ=255)                                 cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt                                          <  2:661719/79‑1 (MQ=255)                                 cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt                                          <  2:661720/79‑1 (MQ=255)                                 cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                                         <  1:661718/80‑1 (MQ=255)                                  aaCCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTaaa                                       >  1:661759/1‑81 (MQ=255)                                     cTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGc                                    >  2:661770/1‑81 (MQ=255)                                         aTGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAATGGCGATAGAAGCAGCCAGTTTAAACGCCAGt                                >  2:661754/1‑81 (MQ=255)                                         aTGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGt                                >  1:661761/1‑81 (MQ=255)                                            aTCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTc                              <  1:661721/80‑1 (MQ=255)                                             tCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCag                            <  2:661722/81‑1 (MQ=255)                                               ggCTCAAGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag                          >  1:661762/1‑81 (MQ=255)                                                     aGCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc                     >  1:661767/1‑80 (MQ=255)                                                      gCAGAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCaa                   >  1:661766/1‑81 (MQ=255)                                                         gAACTGGTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGt                >  2:661771/1‑81 (MQ=255)                                                               gTTTCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATgg           >  1:661763/1‑80 (MQ=255)                                                                  tCGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTaa        <  1:661723/80‑1 (MQ=255)                                                                   cGCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAg       >  1:661758/1‑80 (MQ=255)                                                                    gCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGaa     >  1:661779/1‑81 (MQ=255)                                                                        tCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCg  >  2:661764/1‑80 (MQ=255)                                                                              |                                                                          GTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAACCG  >  NC_000913/3471625‑3471775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑