Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,358	G→A	G80D (GGC→GAC)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,358	0	G	A	100.0%	112.5 / NA	32	G80D (GGC→GAC)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

GAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAT  >  NC_000913/3486282‑3486435                                                                             |                                                                              gAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAg                                                                           >  2:665465/1‑81 (MQ=255)        gTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACg                                                                      >  2:665442/1‑79 (MQ=255)           aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAgc                                                                  <  1:665412/80‑1 (MQ=255)            aGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAgcg                                                                 >  2:665462/1‑80 (MQ=255)               aaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATg                                                             <  1:665413/81‑1 (MQ=255)               aaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATg                                                             >  2:665473/1‑81 (MQ=255)                 tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATggg                                                           >  2:665448/1‑81 (MQ=255)                      ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGt                                                      <  2:665414/81‑1 (MQ=255)                         tcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTg                                                     <  2:665415/79‑1 (MQ=255)                          ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCg                                                   >  1:665453/1‑80 (MQ=255)                           cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGa                                                  <  1:665416/80‑1 (MQ=255)                             aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGaaaa                                               >  2:665499/1‑81 (MQ=255)                             aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGaaaa                                               >  1:665452/1‑81 (MQ=255)                                      aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCtgtg                                      <  2:665417/81‑1 (MQ=255)                                        ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGaa                                    <  1:665418/81‑1 (MQ=255)                                               ttATTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCt                              >  1:665464/1‑80 (MQ=255)                                                 aTTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGa                            >  1:665461/1‑80 (MQ=255)                                                 aTTGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGa                            >  2:665480/1‑80 (MQ=255)                                                  ttGGCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGaaa                          >  1:665437/1‑81 (MQ=255)                                                    ggCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAt                         <  1:665419/80‑1 (MQ=255)                                                     gCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAttt                       <  1:665420/81‑1 (MQ=255)                                                     gCGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAtt                        <  2:665421/80‑1 (MQ=255)                                                      cGAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTc                      <  2:665422/81‑1 (MQ=255)                                                       gAACTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCg                     >  1:665454/1‑81 (MQ=255)                                                        aaCTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCg                     >  1:665458/1‑80 (MQ=255)                                                          cTGGGCCTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                  <  2:665423/81‑1 (MQ=255)                                                                cTGTTTGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaaaaa            >  2:665457/1‑81 (MQ=255)                                                                   tttGAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAttt         >  1:665477/1‑81 (MQ=255)                                                                      gAAGAGGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGc      >  1:665478/1‑81 (MQ=255)                                                                      gAAGAGGACAAGGAAGGTAGCGAATGGGTAAGTGAGAAAAACGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCga      <  1:665424/81‑2 (MQ=255)                                                                         gagGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCaa   >  2:665470/1‑81 (MQ=255)                                                                          agGACCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAt  >  2:665466/1‑81 (MQ=255)                                                                             |                                                                              GAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAT  >  NC_000913/3486282‑3486435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑