Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,487,359	A→G	H190R (CAC→CGC)	yhfK →	putative transporter YhfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,487,359	0	A	G	100.0%	119.3 / NA	35	H190R (CAC→CGC)	yhfK	putative transporter YhfK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

CTGCGCGAGTCACTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCACACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTAT  >  NC_000913/3487283‑3487435                                                                             |                                                                             cTGCGCGAGTCACTAAGTCTGCTGTACTGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCAcc                                                                          >  2:665702/1‑81 (MQ=255) cTGCGCGAGTCACTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCAcc                                                                          >  1:665722/1‑81 (MQ=255)     gcgAGTCACTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGaac                                                                      >  1:665686/1‑79 (MQ=255)        aGTCACTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCt                                                                    >  2:665706/1‑80 (MQ=255)            aCTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGaaaa                                                               <  2:665666/81‑1 (MQ=255)            aCTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGaaa                                                                <  1:665667/80‑1 (MQ=255)              tAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAg                                                             >  2:665724/1‑81 (MQ=255)               aaGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAgc                                                            >  2:665720/1‑81 (MQ=255)                aGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAgcg                                                           >  2:665711/1‑81 (MQ=255)                 gTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAgcgc                                                          <  2:665668/81‑1 (MQ=255)                       tgTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTgccgc                                                    >  1:665738/1‑81 (MQ=255)                        gTACCGTGAACTGGCAGATCATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTgccgcc                                                   >  1:665714/1‑81 (MQ=255)                           ccGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCgct                                                <  2:665669/81‑1 (MQ=255)                               gAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGctgctg                                            >  2:665732/1‑81 (MQ=255)                                 aCTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGt                                          >  1:665721/1‑81 (MQ=255)                                     gCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTgcgc                                      >  2:665719/1‑81 (MQ=255)                                     gCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTgc                                        <  2:665670/79‑1 (MQ=255)                                     gCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTgc                                        <  1:665671/79‑1 (MQ=255)                                       aGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTgcgc                                      >  2:665735/1‑79 (MQ=255)                                           tattGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCa                                 >  1:665727/1‑80 (MQ=255)                                            attGTGAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCaa                                <  2:665672/80‑1 (MQ=255)                                               gtgAAGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAg                            >  1:665728/1‑81 (MQ=255)                                                  aaGCCAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCgg                         >  2:665731/1‑81 (MQ=255)                                                     ccAAATACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCg                      <  2:665673/81‑1 (MQ=255)                                                         aTACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCt                  <  2:665674/81‑1 (MQ=255)                                                         aTACAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCt                  <  1:665675/81‑1 (MQ=255)                                                           aCAGCCTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTaa                >  2:952927/1‑81 (MQ=255)                                                                cTGCTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTAcc           >  2:665734/1‑81 (MQ=255)                                                                   cTTACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCa         <  1:665676/80‑1 (MQ=255)                                                                    ttACCCAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGt       >  1:665733/1‑81 (MQ=255)                                                                       cccAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGc     <  2:665677/80‑1 (MQ=255)                                                                       cccAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCt    <  1:665678/81‑1 (MQ=255)                                                                         cAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTAt  <  2:665680/81‑1 (MQ=255)                                                                         cAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTAt  <  1:665679/81‑1 (MQ=255)                                                                         cAGCGCACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTAt  >  1:665739/1‑81 (MQ=255)                                                                             |                                                                             CTGCGCGAGTCACTAAGTCTGCTGTACCGTGAACTGGCAGATTATTGTGAAGCCAAATACAGCCTGCTTACCCAGCACACCGACCCTGAAAAAGCGCTGCCGCCGCTGCTGGTGCGCCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTAT  >  NC_000913/3487283‑3487435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑