| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,509,874 | T→C | H368R (CAT→CGT) | yhfX ← | putative PLP‑binding protein YhfX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,509,874 | 0 | T | C | 96.8% | 99.7 / ‑3.9 | 31 | H368R (CAT→CGT) | yhfX | putative PLP‑binding protein YhfX |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (15/15); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CACAAATCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGATGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACCACTGCACTGCT > NC_000913/3509799‑3509950 | cacaAATCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATa < 2:671678/81‑1 (MQ=255) aTCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGa < 1:671680/81‑1 (MQ=255) aTCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGa < 1:671679/81‑1 (MQ=255) tCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGac < 1:671681/81‑1 (MQ=255) cgcgCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGaca > 1:671750/1‑81 (MQ=255) cgcgCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGaca > 1:671746/1‑81 (MQ=255) gcgcCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGacac > 2:671733/1‑81 (MQ=255) cgcCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGATGAATACCGGACAcc < 1:671682/81‑1 (MQ=255) cgcCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGacac > 1:671751/1‑80 (MQ=255) aCGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGcacca > 1:671743/1‑81 (MQ=255) gagagTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTAc > 1:671730/1‑81 (MQ=255) agTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACggg > 1:671764/1‑81 (MQ=255) tttCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTg < 1:671683/81‑1 (MQ=255) cAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACaa < 2:671685/81‑1 (MQ=255) cAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACa < 2:671684/80‑1 (MQ=255) aaGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACaaa < 1:671686/81‑1 (MQ=255) tGTCATAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCt > 2:671741/1‑81 (MQ=255) aTAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCtgcgt < 2:671687/81‑1 (MQ=255) aTAACGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCtgcg < 2:671688/80‑1 (MQ=255) aCGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCtgcgtgc > 1:671748/1‑80 (MQ=255) aCGACCGACGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCtgcgtgc < 2:671689/80‑1 (MQ=255) gaccgacGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGaa < 1:671690/81‑1 (MQ=255) gaccgacGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGa > 2:671763/1‑80 (MQ=255) accgacGATTTCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGaa > 1:671749/1‑80 (MQ=255) ttCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGcac > 1:671745/1‑81 (MQ=255) tCCGGTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTGCGGGTGACAAAAATCTGCGTGCGAAAACAGAGcac > 2:671740/1‑80 (MQ=255) gTTCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACcactg > 1:671737/1‑81 (MQ=255) ttCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACcactgc < 2:671691/81‑1 (MQ=255) tCGCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACcactgca > 2:671755/1‑81 (MQ=255) gCCACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACcactgcact < 1:671692/81‑1 (MQ=255) ccACGACGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACcactgcactg < 2:671693/81‑1 (MQ=255) cgacgaATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACCACTGCACTGCt > 2:671760/1‑80 (MQ=255) | CACAAATCGCGCCATTACGCCCCCAGAGAGTTTCCAAGACTGTCATAACGACCGACGATTTCCGGTTCGCCACGATGAATACCGGACACCAGCACCACATCGCTACGGGTGACAAAAATCTGCGTGCGAAAACAGAGCACCACTGCACTGCT > NC_000913/3509799‑3509950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |