Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,521,934	T→C	Q13Q (CAA→CAG)	hofN ←	DNA utilization protein HofN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,521,934	0	T	C	100.0%	154.1 / NA	45	Q13Q (CAA→CAG)	hofN	DNA utilization protein HofN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/23);  total (22/23)

TTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATG  >  NC_000913/3521858‑3522008                                                                             |                                                                           ttAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCgctgct                                                                        <  2:674901/81‑1 (MQ=255) ttAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGc                                                                         <  1:674902/80‑1 (MQ=255)   aGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGctgctg                                                                       <  1:674903/80‑1 (MQ=255)    gCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGt                                                                      <  2:674904/80‑1 (MQ=255)    gCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTc                                                                     <  2:674905/81‑1 (MQ=255)    gCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTc                                                                     <  1:674906/81‑1 (MQ=255)      gTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGc                                                                   >  1:674971/1‑81 (MQ=255)      gTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGc                                                                   >  1:674960/1‑81 (MQ=255)      gTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGc                                                                   >  1:674959/1‑81 (MQ=255)         aTCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCa                                                                 <  1:674907/80‑1 (MQ=255)            ccaaCGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGc                                                             <  1:674909/78‑1 (MQ=255)            ccGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAggg                                                              <  2:674908/80‑1 (MQ=255)              gACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCa                                                            >  2:674973/1‑80 (MQ=255)              gACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCa                                                            >  2:674955/1‑80 (MQ=255)               aCGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCaaa                                                          <  1:674910/81‑1 (MQ=255)               aCGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCaa                                                           >  1:674979/1‑80 (MQ=255)                  gCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCaaaaaa                                                       >  2:674970/1‑81 (MQ=255)                  gCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCaaaaaa                                                       >  2:674975/1‑81 (MQ=255)                   ccAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAaat                                                      <  1:674911/81‑1 (MQ=255)                       cagcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAaattaat                                                  <  1:674912/81‑1 (MQ=255)                        agcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAaattaatt                                                 >  1:674956/1‑81 (MQ=255)                            gagGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCg                                             >  2:674966/1‑81 (MQ=255)                             agGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCAAGGGCAAAAAATTAATTGGCgg                                            >  2:674968/1‑81 (MQ=255)                              ggCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCggg                                           >  1:674996/1‑81 (MQ=255)                               gcgcAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGt                                          >  2:674981/1‑81 (MQ=255)                                 gcAACGAACATCAGCAACCGGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTtc                                        <  1:674913/81‑1 (MQ=255)                                 gcAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGtt                                         >  1:674967/1‑80 (MQ=255)                                   aaCGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTtcat                                      <  1:674914/81‑1 (MQ=255)                                      gAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAg                                   <  2:674915/81‑1 (MQ=255)                                        aCATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTa                                 <  2:674916/81‑1 (MQ=255)                                         cATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTAc                                <  2:674917/81‑1 (MQ=255)                                             aGCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGcc                             <  1:674918/80‑1 (MQ=255)                                              gCAACCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCtt                           >  1:674984/1‑81 (MQ=255)                                                 aCCAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTcc                         >  1:674964/1‑80 (MQ=255)                                                   cAGAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCaa                      <  2:674919/81‑1 (MQ=255)                                                     gAAACGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAgc                    >  2:674963/1‑81 (MQ=255)                                                      aaaCGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAgcg                   >  2:674962/1‑81 (MQ=255)                                                      aaaCGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAgcg                   >  2:674969/1‑81 (MQ=255)                                                        aCGCAGAAAAGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGcc                 <  2:674920/81‑1 (MQ=255)                                                               aaaGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCa          <  2:674921/81‑1 (MQ=255)                                                                 aGCGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAgc        <  1:674922/81‑1 (MQ=255)                                                                   cGGTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAgcgc      >  2:674987/1‑81 (MQ=255)                                                                     gTCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGa    >  2:674980/1‑81 (MQ=255)                                                                      tCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGAt   <  2:674924/81‑1 (MQ=255)                                                                      tCCGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGAt   <  2:674923/81‑1 (MQ=255)                                                                       ccGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATg  >  2:909392/1‑81 (MQ=255)                                                                        cGGCGCTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATg  <  1:674925/80‑1 (MQ=255)                                                                             |                                                                           TTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATG  >  NC_000913/3521858‑3522008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑