| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,568,321 | G→A | G336G (GGC→GGT) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,568,321 | 0 | G | A | 97.8% | 149.7 / ‑4.2 | 45 | G336G (GGC→GGT) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/22); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
ATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGA > NC_000913/3568244‑3568395 | aTGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccaccg < 1:687566/81‑1 (MQ=255) tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg < 2:687567/81‑1 (MQ=255) tGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCgg < 2:687568/81‑1 (MQ=255) ttGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGa < 2:687569/80‑1 (MQ=255) tGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGaaa > 2:687627/1‑81 (MQ=255) cAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAcc < 2:687570/81‑1 (MQ=255) cAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAAc > 1:687621/1‑80 (MQ=255) gaatgaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAg < 2:687571/81‑1 (MQ=255) gaatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAg > 2:687641/1‑81 (MQ=255) aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAg < 2:687572/80‑1 (MQ=255) aatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt > 2:687656/1‑81 (MQ=255) atTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGt > 1:687619/1‑80 (MQ=255) cgcgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAggg < 1:687573/81‑1 (MQ=255) cgAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTc < 2:687574/81‑1 (MQ=255) aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt < 2:687575/81‑1 (MQ=255) aaCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCAt < 1:687576/81‑1 (MQ=255) aCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATc < 1:687577/81‑1 (MQ=255) cgcgcgAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATcc > 1:687646/1‑81 (MQ=255) gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc > 2:687635/1‑80 (MQ=255) gaacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc > 1:687634/1‑80 (MQ=255) aacagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTa < 1:687578/81‑1 (MQ=255) acagaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAc > 1:687660/1‑81 (MQ=255) gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCgg < 2:687579/81‑1 (MQ=255) gaacGGACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCg > 1:687655/1‑80 (MQ=255) ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687676/1‑80 (MQ=255) ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687643/1‑80 (MQ=255) ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGc > 1:687633/1‑80 (MQ=255) ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCg < 1:687580/81‑1 (MQ=255) ggACTGCACCACCACCGAACCGGAGATCACACAACCACCGAAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCg < 1:687581/81‑1 (MQ=255) gACTGCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGa > 1:687644/1‑81 (MQ=255) aCTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGAt < 1:687582/81‑1 (MQ=255) gCACCACCACCGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCt > 1:687628/1‑81 (MQ=255) accaccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGc < 2:687583/81‑1 (MQ=255) accaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCAc > 2:687647/1‑80 (MQ=255) ccaccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGa < 1:687584/81‑1 (MQ=255) caccGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGaa > 2:687636/1‑81 (MQ=255) accGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAt > 2:687650/1‑81 (MQ=255) cGAACCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAAttt > 2:687683/1‑81 (MQ=255) aCCGGAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGc > 2:687661/1‑81 (MQ=255) ggAGATCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTgg > 1:687637/1‑81 (MQ=255) gagaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGc < 1:687585/81‑1 (MQ=255) agaTCACACAACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCg < 1:687586/81‑1 (MQ=255) acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt < 2:687588/81‑1 (MQ=255) acacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAAt < 2:687587/81‑1 (MQ=255) cacaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATg < 1:687589/81‑1 (MQ=255) acaACCACCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGa > 1:687645/1‑81 (MQ=255) | ATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGGAGCGATCCTGCACGAATTTCGCTGGCGGTAATGA > NC_000913/3568244‑3568395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |