Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,417	T→C	A185A (GCT→GCC)	yhhZ →	putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,417	0	T	C	100.0%	63.4 / NA	19	A185A (GCT→GCC)	yhhZ	putative endonuclease YhhZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/11);  total (8/11)

TTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAA  >  NC_000913/3582340‑3582488                                                                              |                                                                        ttACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCcgg                                                                       <  1:691209/80‑1 (MQ=255)  tACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCcggtg                                                                     >  2:691227/1‑81 (MQ=255)     cAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCg                                                                  <  2:691210/81‑1 (MQ=255)       aaTTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGta                                                                <  2:691211/81‑1 (MQ=255)        aTTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGtat                                                               <  2:691212/81‑1 (MQ=255)           cAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATAc                                                             <  1:691213/80‑1 (MQ=255)            aaaaaaCCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTACTGCCGGTGGCGTATACaa                                                           >  1:691238/1‑81 (MQ=255)                      acaaaTAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACAtt                                                 >  1:691231/1‑81 (MQ=255)                          ataatCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAg                                             >  1:691235/1‑81 (MQ=255)                                gTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTc                                        <  2:691214/80‑1 (MQ=255)                                 tttCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCag                                      <  2:691215/81‑1 (MQ=255)                                   tCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCa                                       >  1:691234/1‑78 (MQ=255)                                    cAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCagag                                    <  2:691216/80‑1 (MQ=255)                                      aaaCGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATa                                 >  1:691228/1‑81 (MQ=255)                                               aCAGCAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGaa                         >  2:691229/1‑80 (MQ=255)                                                   cAAAGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACt                    <  1:691217/81‑1 (MQ=255)                                                     aaGCTATTGGCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTgg                  >  1:691237/1‑81 (MQ=255)                                                             ggCAGGCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGgaga           <  1:691218/80‑1 (MQ=255)                                                                  gCTACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAAt     <  2:691219/81‑1 (MQ=255)                                                                    tACTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATa    >  1:691240/1‑80 (MQ=255)                                                                      cTTGCTGCCGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATaaa  <  1:691220/80‑1 (MQ=255)                                                                              |                                                                        TTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAA  >  NC_000913/3582340‑3582488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑