Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,590,742	T→C	D57G (GAT→GGT)	ugpA ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,590,742	0	T	C	100.0%	161.8 / NA	46	D57G (GAT→GGT)	ugpA	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

GTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGC  >  NC_000913/3590664‑3590819                                                                               |                                                                              gTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTAccc                                                                             <  1:693191/81‑1 (MQ=255) gTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTAccc                                                                             <  2:693190/81‑1 (MQ=255)     cAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGc                                                                         >  1:695335/1‑81 (MQ=255)      aaaGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGcc                                                                        >  2:693248/1‑81 (MQ=255)        aGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGa                                                                      <  1:693192/81‑1 (MQ=255)               gAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActg                                                               >  2:693262/1‑81 (MQ=255)                aaTTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActgg                                                              >  1:693281/1‑81 (MQ=255)                aaTTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActgg                                                              <  2:693194/81‑1 (MQ=255)                aaTTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActg                                                               <  2:693193/80‑1 (MQ=255)                  ttttATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggct                                                            <  2:693195/81‑1 (MQ=255)                   tttATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggctg                                                           >  1:693246/1‑81 (MQ=255)                   tttATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggctg                                                           >  1:693251/1‑81 (MQ=255)                    ttATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggctg                                                           <  2:693196/80‑1 (MQ=255)                     tATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggctgg                                                          <  2:693197/80‑1 (MQ=255)                     tATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAActggctg                                                           >  1:693252/1‑79 (MQ=255)                          tcgtcCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAAc                                                    >  1:693274/1‑81 (MQ=255)                           cgtcCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAAc                                                    >  2:693257/1‑80 (MQ=255)                           cgtcCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAAc                                                    >  2:693254/1‑80 (MQ=255)                            gtcCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAAcc                                                   >  2:693266/1‑80 (MQ=255)                            gtcCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAAcc                                                   >  2:693268/1‑80 (MQ=255)                              ccAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCaaa                                                >  2:693265/1‑81 (MQ=255)                              ccAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCaaa                                                >  1:693277/1‑81 (MQ=255)                               cAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAAc                                               <  1:693198/81‑1 (MQ=255)                               cAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAAc                                               <  2:693199/81‑1 (MQ=255)                               cAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAAc                                               >  2:693267/1‑81 (MQ=255)                                aGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACg                                              <  1:693200/81‑1 (MQ=255)                                    ggAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATc                                          >  1:693269/1‑81 (MQ=255)                                    ggAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATc                                          >  1:693260/1‑81 (MQ=255)                                    ggAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATc                                          >  1:693250/1‑81 (MQ=255)                                     gAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCg                                         <  2:693201/81‑1 (MQ=255)                                      aGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGa                                        <  1:693202/81‑1 (MQ=255)                                      aGTCGAGATAGTAGCTGTCATGGAACAGCGTAACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGa                                        <  2:693203/81‑1 (MQ=255)                                       gTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGAc                                       <  2:693204/81‑1 (MQ=255)                                          gagaTAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCt                                    <  1:693205/81‑1 (MQ=255)                                             aTAGTAGCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTg                                 >  2:693273/1‑81 (MQ=255)                                                gtagCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTa                               <  1:693206/80‑1 (MQ=255)                                                   gCTGTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGc                             >  1:693259/1‑79 (MQ=255)                                                      gTCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTa                        >  2:693271/1‑81 (MQ=255)                                                       tCATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTa                        <  2:693207/80‑1 (MQ=255)                                                        cATGGAACAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTAc                       >  2:693258/1‑80 (MQ=255)                                                             aaCAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAAc                 <  1:693209/81‑1 (MQ=255)                                                             aaCAGCGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAAc                 <  1:693208/81‑1 (MQ=255)                                                                  cGTGACGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTc            <  2:693210/81‑1 (MQ=255)                                                                      aCGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGccc        <  1:693211/81‑1 (MQ=255)                                                                       cGAAGTTACTCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCg       >  1:693263/1‑81 (MQ=255)                                                                       cGAAGTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCg       <  2:693212/81‑1 (MQ=255)                                                                           gTTACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAgg   >  1:693278/1‑81 (MQ=255)                                                                            ttACCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGc  <  1:693213/81‑1 (MQ=255)                                                                               |                                                                              GTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGC  >  NC_000913/3590664‑3590819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑