Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,611,584	A→G	F50F (TTT→TTC)	yhhS ←	putative transporter YhhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,611,584	0	A	G	100.0%	119.9 / NA	35	F50F (TTT→TTC)	yhhS	putative transporter YhhS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

TAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG  >  NC_000913/3611507‑3611656                                                                              |                                                                         tAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAg                                                                       <  2:699009/81‑1 (MQ=255)     gTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCca                                                                   >  1:699049/1‑81 (MQ=255)       ccGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcat                                                                  >  1:699075/1‑80 (MQ=255)       ccGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcat                                                                  >  1:699076/1‑80 (MQ=255)            aTGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcatcacat                                                             >  1:699052/1‑80 (MQ=255)            aTGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCcatcacat                                                             >  1:699053/1‑80 (MQ=255)              gAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCAcatcat                                                          >  2:699118/1‑81 (MQ=255)                gggCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATgg                                                        <  2:699010/81‑1 (MQ=255)                  gcggcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGAc                                                      <  2:699011/81‑1 (MQ=255)                         aGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGccc                                               <  1:699012/81‑1 (MQ=255)                         aGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGccc                                               >  2:699078/1‑81 (MQ=255)                             aGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCTGt                                            >  2:699080/1‑80 (MQ=255)                                 ggCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATa                                        >  1:699088/1‑80 (MQ=255)                                  gCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATAc                                       <  2:964682/80‑1 (MQ=255)                                   cGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACag                                     <  1:699013/81‑1 (MQ=255)                                   cGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACag                                     >  2:699062/1‑81 (MQ=255)                                      aaTATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcga                                  <  1:699014/81‑1 (MQ=255)                                         atTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACagcgagcg                               >  1:699066/1‑81 (MQ=255)                                            gCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGc                             <  1:699015/80‑1 (MQ=255)                                            gCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGc                             >  2:699057/1‑80 (MQ=255)                                              aGGCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAc                          >  1:699092/1‑81 (MQ=255)                                               ggCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAcc                         <  1:699016/81‑1 (MQ=255)                                               ggCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAcc                         <  1:699017/81‑1 (MQ=255)                                                gCTGATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAAccc                        <  1:699018/81‑1 (MQ=255)                                                   gATAACCAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGAt                     >  2:699071/1‑81 (MQ=255)                                                        ccAATCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACCGCGAGCGGCAACCCGATGGTGa                <  1:699019/81‑1 (MQ=255)                                                            tCCTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTa            <  1:699020/81‑1 (MQ=255)                                                             ccTGCCCAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAg           >  2:699089/1‑81 (MQ=255)                                                                 cccAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTgg       >  1:699070/1‑81 (MQ=255)                                                                  ccAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGc      >  1:699081/1‑81 (MQ=255)                                                                  ccAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGc      >  2:699083/1‑81 (MQ=255)                                                                   cAGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCg     <  2:699021/81‑1 (MQ=255)                                                                    aGAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCg     >  2:699099/1‑80 (MQ=255)                                                                     gAAGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCg     >  1:699087/1‑79 (MQ=255)                                                                      aaGGCGCTGAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAg  >  2:699069/1‑81 (MQ=255)                                                                              |                                                                         TAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAG  >  NC_000913/3611507‑3611656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑