Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,626,245	T→C	T187A (ACC→GCC)	yhhJ ←	ABC transporter family protein YhhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,626,245	0	T	C	100.0%	164.3 / NA	47	T187A (ACC→GCC)	yhhJ	ABC transporter family protein YhhJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/18);  total (29/18)

TCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTCC  >  NC_000913/3626167‑3626321                                                                               |                                                                             tCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCaa                                                                            <  2:703177/81‑1 (MQ=255)   aCCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAAt                                                                           <  1:703178/80‑1 (MQ=255)        tAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATgttgtt                                                                     <  1:703180/81‑1 (MQ=255)        tAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATgttgtt                                                                     <  2:703179/81‑1 (MQ=255)           gtgtTCCACCGTGCCGTGTTCACGCTGGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTtgat                                                                  <  1:703181/81‑1 (MQ=255)           gtgtTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTtgat                                                                  <  1:703182/81‑1 (MQ=255)           gtgtTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTtgat                                                                  >  1:703234/1‑81 (MQ=255)              ttCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTgatgat                                                               <  2:703183/81‑1 (MQ=255)                ccACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCg                                                             >  2:703241/1‑81 (MQ=255)                ccACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCg                                                             >  2:703211/1‑81 (MQ=255)                 cACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCg                                                             <  1:703184/80‑1 (MQ=255)                 cACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGc                                                            >  2:703214/1‑81 (MQ=255)                 cACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGc                                                            >  2:703218/1‑81 (MQ=255)                  aCCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGc                                                            >  2:703212/1‑80 (MQ=255)                             ttCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGc                                                 >  1:703222/1‑80 (MQ=255)                               cACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCa                                               <  1:703185/80‑1 (MQ=255)                                  gCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAAc                                            >  1:703228/1‑80 (MQ=255)                                     cgcgGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCAcg                                        >  2:703246/1‑81 (MQ=255)                                     cgcgGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCAcg                                        >  1:703247/1‑81 (MQ=255)                                        ggATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCgg                                     >  2:703245/1‑81 (MQ=255)                                        ggATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCgg                                     >  1:703219/1‑81 (MQ=255)                                        ggATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCgg                                     <  1:703186/81‑1 (MQ=255)                                         gATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCgg                                     >  2:703260/1‑80 (MQ=255)                                             aGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGagc                                 >  2:703248/1‑78 (MQ=255)                                             aGCGCCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATc                                 >  1:703255/1‑80 (MQ=255)                                               cgcCGATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAg                              >  1:703227/1‑81 (MQ=255)                                                   gATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTc                          >  2:703268/1‑81 (MQ=255)                                                   gATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTc                          >  2:703251/1‑81 (MQ=255)                                                   gATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTc                          <  2:703188/81‑1 (MQ=255)                                                   gATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTc                          >  1:703254/1‑81 (MQ=255)                                                   gATCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTc                          <  1:703187/81‑1 (MQ=255)                                                     tCCGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCgg                        >  2:703238/1‑81 (MQ=255)                                                      ccGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCggg                       >  2:703226/1‑81 (MQ=255)                                                       cGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTTGGGt                      >  1:703257/1‑81 (MQ=255)                                                       cGGTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCggg                       <  2:703189/80‑1 (MQ=255)                                                        ggTCAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGt                      >  1:703230/1‑80 (MQ=255)                                                          tCAATACAATCGCCAGCATGGCAATGATGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTaa                   <  2:703190/81‑1 (MQ=255)                                                           cAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTa                    >  2:703264/1‑79 (MQ=255)                                                           cAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTa                    >  2:703240/1‑79 (MQ=255)                                                           cAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTa                    >  2:703243/1‑79 (MQ=255)                                                           cAATACAATCGCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTa                    >  1:703235/1‑79 (MQ=255)                                                                     gCCAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCgg        <  1:703191/81‑1 (MQ=255)                                                                       cAGCATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGt      >  2:703242/1‑81 (MQ=255)                                                                          cATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTc   <  2:703192/81‑1 (MQ=255)                                                                          cATGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTc   >  2:703233/1‑81 (MQ=255)                                                                           aTGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTcc  <  2:703194/81‑1 (MQ=255)                                                                           aTGGCAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTcc  <  1:703193/81‑1 (MQ=255)                                                                               |                                                                             TCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATCGAGGTTCGGGTTAAAGCGCATCCGGGTTTCC  >  NC_000913/3626167‑3626321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑