Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,645,592	C→T	Q70* (CAG→TAG)	rlmJ →	23S rRNA m(6)A2030 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,645,592	0	C	T	100.0%	170.5 / NA	47	Q70* (CAG→TAG)	rlmJ	23S rRNA m(6)A2030 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/20);  total (27/20)

GCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCG  >  NC_000913/3645517‑3645668                                                                            |                                                                             gCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGtagcag                                                                         >  2:707970/1‑81 (MQ=255) gCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGtagcag                                                                         >  1:707957/1‑81 (MQ=255)  ccGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGtagcag                                                                         >  2:707967/1‑80 (MQ=255)        gTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGAtt                                                                  <  2:707905/81‑1 (MQ=255)          tATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTg                                                                >  1:707960/1‑81 (MQ=255)              aGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGccc                                                             <  1:707907/80‑1 (MQ=255)              aGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGccc                                                             <  1:707906/80‑1 (MQ=255)                  aGGCAGCGAACATGCCTAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAg                                                         >  2:707908/1‑80 (MQ=255)                   ggCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGa                                                        <  2:707910/80‑1 (MQ=255)                   ggCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGa                                                        <  2:707909/80‑1 (MQ=255)                     cAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACt                                                     <  2:707911/81‑1 (MQ=255)                     cAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACt                                                     >  2:707987/1‑81 (MQ=255)                      aGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTg                                                    <  1:707912/81‑1 (MQ=255)                       gCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTg                                                    >  2:707965/1‑80 (MQ=255)                         gAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAg                                                 >  2:707998/1‑81 (MQ=255)                            cATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCg                                              <  1:707913/81‑1 (MQ=255)                             aTGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCg                                              >  2:707975/1‑80 (MQ=255)                                  gAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACAt                                         >  2:707999/1‑80 (MQ=255)                                   aGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATc                                        >  2:707980/1‑80 (MQ=255)                                      gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtg                                    >  1:707981/1‑81 (MQ=255)                                      gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtg                                    >  2:708000/1‑81 (MQ=255)                                      gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtg                                    >  2:707989/1‑81 (MQ=255)                                       tACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtgt                                   >  1:707988/1‑81 (MQ=255)                                        aCCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtgtg                                  >  2:707985/1‑81 (MQ=255)                                           ggCGAATATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGt                                >  1:707968/1‑80 (MQ=255)                                               aaTATCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTaaaa                            >  1:708004/1‑80 (MQ=255)                                                atatCTCGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAaca                          >  2:707991/1‑81 (MQ=255)                                                   tctcGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACt                        <  2:707914/80‑1 (MQ=255)                                                    ctcGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACtt                       >  2:707977/1‑80 (MQ=255)                                                      cGAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCaa                    >  2:707986/1‑81 (MQ=255)                                                       gAAGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCa                     <  1:707915/79‑1 (MQ=255)                                                        aaGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAAcc                  <  2:707916/81‑1 (MQ=255)                                                        aaGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAAcc                  >  1:708005/1‑81 (MQ=255)                                                         aGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCg                 <  1:707917/81‑1 (MQ=255)                                                         aGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCg                 <  1:707918/81‑1 (MQ=255)                                                         aGGCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCg                 >  2:707992/1‑81 (MQ=255)                                                          ggCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCg                 >  1:708014/1‑80 (MQ=255)                                                          ggCATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGt                <  2:707919/81‑1 (MQ=255)                                                            cATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAg              >  2:707974/1‑81 (MQ=255)                                                            cATCGCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAg              <  1:707920/81‑1 (MQ=255)                                                                gCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGt          >  2:707995/1‑81 (MQ=255)                                                                gCCCGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGt          >  2:707973/1‑81 (MQ=255)                                                                   cGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAg       <  2:707921/81‑1 (MQ=255)                                                                   cGTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAg       >  2:707979/1‑81 (MQ=255)                                                                    gTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGt      <  2:707923/81‑1 (MQ=255)                                                                    gTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGt      <  2:707924/81‑1 (MQ=255)                                                                    gTATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGt      <  1:707922/81‑1 (MQ=255)                                                                     tATCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGtt     <  1:707925/81‑1 (MQ=255)                                                                      aTCTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTg    >  2:708019/1‑81 (MQ=255)                                                                        cTGGTAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCg  >  1:707976/1‑81 (MQ=255)                                                                            |                                                                             GCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCG  >  NC_000913/3645517‑3645668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑