Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,658,642	G→A	E93K (GAA→AAA)	gadE →	DNA‑binding transcriptional activator GadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,642	0	G	A	100.0%	42.7 / NA	13	E93K (GAA→AAA)	gadE	DNA‑binding transcriptional activator GadE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

TTGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATG  >  NC_000913/3658574‑3658709                                                                     |                                                                    ttGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCaaa                                                          <  2:710554/80‑1 (MQ=255) ttGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCaaa                                                          >  2:710589/1‑80 (MQ=255)     tttGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAAtct                                                     >  2:710572/1‑81 (MQ=255)      ttGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAAtctt                                                    >  1:710575/1‑81 (MQ=255)                aTCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTa                                          >  2:710567/1‑81 (MQ=255)                   acaTCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACt                                        <  2:710555/80‑1 (MQ=255)                          tAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTa                                 >  2:710577/1‑80 (MQ=255)                                       aaaGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCa                    >  2:710579/1‑80 (MQ=255)                                       aaaGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCa                    >  2:710582/1‑80 (MQ=255)                                        aaGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCAt                   <  2:710557/80‑1 (MQ=255)                                        aaGCGCCTATTTATTTTGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATa                  <  1:710556/81‑1 (MQ=255)                                                       ttGTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTAt   >  1:710571/1‑81 (MQ=255)                                                         gTTAGCCGCAAAAAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATg  <  2:710558/80‑1 (MQ=255)                                                                     |                                                                    TTGTTTTGGCACCTTATCACATCAGTAAACTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATG  >  NC_000913/3658574‑3658709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑