Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,674,704	T→C	intergenic (+329/‑82)	yhjD → / → yhjE	putative transporter YhjD/putative transporter YhjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,674,704	0	T	C	100.0%	96.6 / NA	28	intergenic (+329/‑82)	yhjD/yhjE	putative transporter YhjD/putative transporter YhjE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/17);  total (11/17)

AAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAA  >  NC_000913/3674628‑3674779                                                                             |                                                                            aaaTTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCcg                                                                         <  1:714384/81‑1 (MQ=255) aaaTTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCcg                                                                         >  2:714425/1‑81 (MQ=255)  aaTTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCcgc                                                                        <  1:714385/81‑1 (MQ=255)  aaTTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCcgc                                                                        <  1:714386/81‑1 (MQ=255)       tgtgAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGAtt                                                                    <  2:714387/80‑1 (MQ=255)         tgAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGt                                                                 <  1:714388/81‑1 (MQ=255)            aGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTac                                                              <  1:714389/81‑1 (MQ=255)               aTTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTAcaca                                                           <  1:714390/81‑1 (MQ=255)                  tCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACAtt                                                         >  1:714424/1‑80 (MQ=255)                   cATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACAttt                                                        <  2:714391/80‑2 (MQ=255)                      agaagaAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTaaa                                                     >  1:714411/1‑80 (MQ=255)                       gaagaaAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAata                                                   <  2:714392/81‑1 (MQ=255)                           aaaaaTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCAc                                               >  2:714421/1‑81 (MQ=255)                              aaTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACttt                                            <  1:714393/81‑1 (MQ=255)                               aTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACtttt                                           <  2:714394/81‑1 (MQ=255)                                     ggCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCtgtg                                     >  2:714420/1‑81 (MQ=255)                                      gCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGc                                    >  1:714416/1‑81 (MQ=255)                                               ttATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATgg                           <  1:714395/81‑1 (MQ=255)                                                  tCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTa                        >  1:714447/1‑81 (MQ=255)                                                     ccTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTc                     >  1:714419/1‑81 (MQ=255)                                                     ccTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTc                     <  2:714396/81‑1 (MQ=255)                                                           tGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGc               >  2:714423/1‑81 (MQ=255)                                                                 aTTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaaaaa         <  2:714397/81‑1 (MQ=255)                                                                   ttttGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaaaaaa        >  1:714428/1‑80 (MQ=255)                                                                     ttGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAAt       >  1:714454/1‑79 (MQ=255)                                                                      tGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATaa     <  2:714398/80‑1 (MQ=255)                                                                        aCCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGaa  <  1:714400/81‑1 (MQ=255)                                                                        aCCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGa   <  1:714399/80‑1 (MQ=255)                                                                             |                                                                            AAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAA  >  NC_000913/3674628‑3674779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑