Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	43,173	A→G	*257* (TAA→TAG)	fixA →	putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	43,173	0	A	G	100.0%	116.6 / NA	34	*257* (TAA→TAG)	fixA	putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

AGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACT  >  NC_000913/43100‑43246                                                                          |                                                                          aGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAgg                                                                    >  2:10326/1‑81 (MQ=255) aGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAgg                                                                    <  1:10275/81‑1 (MQ=255) aGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCCGAAAATCTTCGCAAAGTCATTTAGTTACAgg                                                                    >  2:10329/1‑81 (MQ=255)         gTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTa                                                            >  2:10331/1‑81 (MQ=255)                aaGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAacac                                                     >  1:10345/1‑81 (MQ=255)                  ggCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGt                                                   <  2:10276/81‑1 (MQ=255)                    cGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGttt                                                 >  1:10330/1‑81 (MQ=255)                    cGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGttt                                                 <  2:10277/81‑1 (MQ=255)                     gACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGtttt                                                >  1:10346/1‑81 (MQ=255)                      aCGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTtc                                               <  1:10278/81‑1 (MQ=255)                      aCGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTtc                                               <  2:10279/81‑1 (MQ=255)                        ggCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTtctc                                             <  2:10280/81‑1 (MQ=255)                        ggCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTtctc                                             <  2:10281/81‑1 (MQ=255)                          cGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCaa                                           <  1:10282/81‑1 (MQ=255)                           gaagaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAg                                          >  1:10372/1‑81 (MQ=255)                            aagaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGt                                         >  2:10339/1‑81 (MQ=255)                             agaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTc                                        <  2:10283/81‑1 (MQ=255)                              gaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCt                                       <  2:10285/81‑1 (MQ=255)                              gaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCt                                       <  1:10284/81‑1 (MQ=255)                                aCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTgg                                     >  1:10350/1‑81 (MQ=255)                                 cAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTggg                                    <  2:10286/81‑1 (MQ=255)                                   gATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTa                                  >  2:10344/1‑81 (MQ=255)                                     tCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTAtt                                >  2:10349/1‑81 (MQ=255)                                     tCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTAtt                                >  2:10340/1‑81 (MQ=255)                                     tCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTAtt                                <  1:10287/81‑1 (MQ=255)                                         cgcATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGc                            >  1:10338/1‑81 (MQ=255)                                           cATTTGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGa                          <  2:10288/81‑1 (MQ=255)                                               tGCTGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATAc                       <  2:10289/80‑1 (MQ=255)                                                  tGAAAATCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCt                   >  1:10347/1‑81 (MQ=255)                                                       aTCTTCGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCg              >  1:10343/1‑81 (MQ=255)                                                            cGCAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGt             <  1:10290/77‑1 (MQ=255)                                                              cAAAGTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCg       <  2:10291/81‑1 (MQ=255)                                                                  gTCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAAc   >  2:10342/1‑81 (MQ=255)                                                                   tCATTTAGTTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACt  <  2:10292/81‑1 (MQ=255)                                                                          |                                                                          AGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACT  >  NC_000913/43100‑43246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑