Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	387,413	T→A	L148Q (CTG→CAG)	tauC →	taurine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	387,413	0	T	A	100.0%	98.4 / NA	28	L148Q (CTG→CAG)	tauC	taurine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/15);  total (13/15)

GTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGTTCG  >  NC_000913/387338‑387485                                                                            |                                                                         gTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATc                                                                      <  2:87866/80‑1 (MQ=255)    cGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTAt                                                                   <  2:87867/80‑1 (MQ=255)        gccgccGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGc                                                              >  1:87921/1‑81 (MQ=255)        gccgccGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGc                                                              >  2:87914/1‑81 (MQ=255)           gccgcTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAt                                                           <  2:87868/81‑1 (MQ=255)            ccgcTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAtt                                                          <  2:87869/81‑1 (MQ=255)             cgcTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAttt                                                         >  1:87912/1‑81 (MQ=255)                tGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTg                                                      >  2:87910/1‑81 (MQ=255)                   cTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCAc                                                   <  1:87870/81‑1 (MQ=255)                      aTTTGCCGCTGATGGTGATCTGGTTTGGTATTAGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCAcc                                                  <  1:87871/79‑1 (MQ=255)                         tGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTgg                                             >  1:87922/1‑81 (MQ=255)                           ccGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGc                                            >  1:87930/1‑80 (MQ=255)                             gCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGAt                                         >  1:87924/1‑81 (MQ=255)                              cTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATg                                        <  1:87872/81‑1 (MQ=255)                                 aTGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTc                                      <  1:87873/80‑1 (MQ=255)                                     tGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGgcgc                                 <  2:87874/81‑1 (MQ=255)                                      gATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCt                                >  1:87903/1‑81 (MQ=255)                                       aTCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCt                                <  1:87875/80‑1 (MQ=255)                                         cTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGc                             >  1:87919/1‑81 (MQ=255)                                            gTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTg                               <  1:87877/76‑1 (MQ=255)                                            gTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGc                             <  2:87876/78‑1 (MQ=255)                                            gTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCggg                          <  2:87878/81‑1 (MQ=255)                                                ggTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGt                       <  2:87879/80‑1 (MQ=255)                                                   aTTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGaa                    >  2:87928/1‑80 (MQ=255)                                                       gTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCg               >  1:87927/1‑81 (MQ=255)                                                          aaaCCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTg             >  1:87929/1‑80 (MQ=255)                                                            aCCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTgcag          >  2:87915/1‑81 (MQ=255)                                                                    gATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGGGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGTTCg  <  1:87880/81‑1 (MQ=255)                                                                            |                                                                         GTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGTTCG  >  NC_000913/387338‑387485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑