Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,674,725	G→T	intergenic (+350/‑61)	yhjD → / → yhjE	putative transporter YhjD/putative transporter YhjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,674,725	0	G	T	100.0%	86.2 / NA	25	intergenic (+350/‑61)	yhjD/yhjE	putative transporter YhjD/putative transporter YhjE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/10);  total (15/10)

AGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAA  >  NC_000913/3674649‑3674795                                                                             |                                                                       agaagaAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTaaa                                                                     >  1:714411/1‑80 (MQ=255)  gaagaaAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAata                                                                   <  2:714392/81‑1 (MQ=255)      aaaaaTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCAc                                                               >  2:714421/1‑81 (MQ=255)         aaTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACttt                                                            <  1:714393/81‑1 (MQ=255)          aTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACtttt                                                           <  2:714394/81‑1 (MQ=255)                ggCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCtgtg                                                     >  2:714420/1‑81 (MQ=255)                 gCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGc                                                    >  1:714416/1‑81 (MQ=255)                          ttATCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATgg                                           <  1:714395/81‑1 (MQ=255)                             tCCCCTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTa                                        >  1:714447/1‑81 (MQ=255)                                ccTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTc                                     >  1:714419/1‑81 (MQ=255)                                ccTTTTTGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTc                                     <  2:714396/81‑1 (MQ=255)                                      tGCCTGATTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGc                               >  2:714423/1‑81 (MQ=255)                                            aTTTTTGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaaaaa                         <  2:714397/81‑1 (MQ=255)                                              ttttGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaaaaaa                        >  1:714428/1‑80 (MQ=255)                                                ttGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAAt                       >  1:714454/1‑79 (MQ=255)                                                 tGACCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATaa                     <  2:714398/80‑1 (MQ=255)                                                   aCCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGaa                  <  1:714400/81‑1 (MQ=255)                                                   aCCATCTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGa                   <  1:714399/80‑1 (MQ=255)                                                      atctCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGaa                  >  2:714431/4‑78 (MQ=255)                                                            gcgATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATg         >  1:714440/1‑81 (MQ=255)                                                              gATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgc        >  2:714433/1‑80 (MQ=255)                                                                tttGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaag     >  1:714436/1‑81 (MQ=255)                                                                  tGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaagc    >  2:714426/1‑80 (MQ=255)                                                                    ttACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaagcaa  <  2:714401/80‑1 (MQ=255)                                                                     tACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaagcaa  >  2:714430/1‑79 (MQ=255)                                                                             |                                                                       AGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAA  >  NC_000913/3674649‑3674795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑