| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,704,577 | C→T | R62H (CGC→CAC) | dppC ← | dipeptide ABC transporter membrane subunit DppC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,704,577 | 0 | C | T | 98.2% | 195.3 / ‑4.5 | 55 | R62H (CGC→CAC) | dppC | dipeptide ABC transporter membrane subunit DppC |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (25/29); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
GGCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGCGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGACGACGTAAAC > NC_000913/3704500‑3704653 | ggCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGa < 1:722673/81‑1 (MQ=255)ggCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGa < 2:722674/81‑1 (MQ=255)ggCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGa < 2:722672/81‑1 (MQ=255) gCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGaa < 2:722675/81‑1 (MQ=255) ccTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAAc < 1:722676/81‑1 (MQ=255) cTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACt < 1:722677/81‑1 (MQ=255) cTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACt > 2:722737/1‑81 (MQ=255) aCGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGt > 2:722746/1‑81 (MQ=255) tCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTcc < 2:722678/81‑1 (MQ=255) ggTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCgg > 2:722719/1‑81 (MQ=255) gTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCggg < 1:722679/81‑1 (MQ=255) gCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGt > 2:722736/1‑80 (MQ=255) cccAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTta > 1:722766/1‑81 (MQ=255) cccAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTta < 1:722681/81‑1 (MQ=255) cacAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGtt < 2:722680/78‑1 (MQ=255) ccAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTtat < 1:722682/81‑1 (MQ=255) cAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTtata < 2:722683/81‑1 (MQ=255) cAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTta > 2:722743/1‑79 (MQ=255) cAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAggg < 2:722684/81‑1 (MQ=255) aaGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGt > 1:722738/1‑81 (MQ=255) aGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTg > 1:722735/1‑81 (MQ=255) gTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTg > 1:722772/1‑80 (MQ=255) gTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTg > 1:722740/1‑80 (MQ=255) ttcgcCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGc > 1:722773/3‑80 (MQ=255) tGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCa < 2:722685/81‑1 (MQ=255) gcCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATc < 1:722686/81‑1 (MQ=255) cATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCa < 2:722687/81‑1 (MQ=255) tgctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGt < 2:722688/81‑1 (MQ=255) tgctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGt < 1:722690/81‑1 (MQ=255) tgctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGt < 2:722689/81‑1 (MQ=255) gctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGtt > 2:722758/1‑81 (MQ=255) ctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTg < 1:722691/81‑1 (MQ=255) ctgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTg > 2:722744/1‑81 (MQ=255) tgcCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTgg > 2:722749/1‑81 (MQ=255) gccTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCa > 1:722768/1‑79 (MQ=255) gccTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCa > 2:722759/1‑79 (MQ=255) ccTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATcg > 1:722769/1‑81 (MQ=255) ccTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATc < 1:722692/80‑1 (MQ=255) cTGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATcgc > 2:722764/1‑81 (MQ=255) tGCCAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATcgc > 2:722757/1‑80 (MQ=255) ccAGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGAt < 1:722693/81‑1 (MQ=255) aGGCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATg > 1:722752/1‑80 (MQ=255) ggCTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGaa < 2:722694/81‑1 (MQ=255) cTGGCGGGGCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACa < 2:722695/81‑1 (MQ=255) gggCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGa > 2:722776/1‑81 (MQ=255) ggCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGAt > 2:722824/1‑81 (MQ=255) gCGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATg < 1:722696/81‑1 (MQ=255) cGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATga > 1:722753/1‑81 (MQ=255) cGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATga > 2:722751/1‑81 (MQ=255) cGAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATga < 1:722697/81‑1 (MQ=255) gAGCAGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATgac < 2:722698/81‑1 (MQ=255) aGTGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGacgacg > 2:722767/1‑81 (MQ=255) aGTGCATCGCGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGacgacg > 2:722756/1‑81 (MQ=255) tGCATCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGACGACGTa < 1:722699/81‑1 (MQ=255) aTCGTGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGACGACGTAAAc < 2:722700/81‑1 (MQ=255) | GGCCTACGTCATCGGTGCCCAGCAAGTGCGCCATGCTGCCGCCTTCCTGCCAGGCTGGCGGGGCGAGCAGTGCATCGCGGAACTGTTCCGCCGGGTTATAGGGTGCAATCCAGTTGGCAAAGATCGCGATGAACAGCACGATGACGACGTAAAC > NC_000913/3704500‑3704653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |