| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,704,816 | A→T | D325E (GAT→GAA) | dppB ← | dipeptide ABC transporter membrane subunit DppB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,704,816 | 0 | A | T | 98.0% | 175.4 / ‑3.7 | 50 | D325E (GAT→GAA) | dppB | dipeptide ABC transporter membrane subunit DppB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (26/23); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGATCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTCGCGGC > NC_000913/3704740‑3704892 | tATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTc < 1:722755/79‑1 (MQ=255)tATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGa > 2:722819/1‑81 (MQ=255) aTTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGATCGa < 1:722756/80‑1 (MQ=255) ttttCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGAcc < 1:722757/81‑1 (MQ=255) tttCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGAc > 2:722818/1‑79 (MQ=255) aGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGc > 2:722803/1‑79 (MQ=255) aGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGc > 1:722829/1‑79 (MQ=255) tAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGt < 1:722758/81‑1 (MQ=255) aCCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGtt > 1:722815/1‑80 (MQ=255) tgACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAg < 1:722759/81‑1 (MQ=255) atgatgTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGAt > 2:722816/1‑80 (MQ=255) atgatgTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATa < 1:722760/81‑1 (MQ=255) gatgTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAat > 1:722831/1‑81 (MQ=255) tgTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatca > 2:722830/1‑81 (MQ=255) tCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatcatc < 2:722762/81‑1 (MQ=255) tCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatcatc < 2:722761/81‑1 (MQ=255) tCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatcatc < 1:722763/81‑1 (MQ=255) tCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatcatc < 1:722764/81‑1 (MQ=255) tCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAatcat < 1:722765/80‑1 (MQ=255) ccccTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCAtcg > 1:722875/1‑81 (MQ=255) cccTTACTTCTTATGACGAATACGCGGGTTCACTACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCAtcgt > 1:722845/1‑81 (MQ=255) ccTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCAtcgtc < 2:722766/81‑1 (MQ=255) aCTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGcc < 2:722768/80‑1 (MQ=255) cttATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGccacca < 2:722769/80‑1 (MQ=255) tATGACGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGc > 1:722835/1‑80 (MQ=255) aCGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAc < 2:722770/81‑1 (MQ=255) aCGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAc > 2:722836/1‑81 (MQ=255) aCGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAc > 2:722838/1‑81 (MQ=255) cGAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcg < 1:722771/81‑1 (MQ=255) gAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcgc < 2:722772/81‑1 (MQ=255) gAATACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcgc > 2:722859/1‑81 (MQ=255) aaTACGCGGGTTCACCACGCTGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcgcc < 2:722773/81‑1 (MQ=255) aaTACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcgcc > 2:722820/1‑81 (MQ=255) tACGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATAcgccgc > 1:722825/1‑81 (MQ=255) aCGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACgccgcc > 2:722840/1‑81 (MQ=255) aCGCGGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACgccgcc > 1:722822/1‑81 (MQ=255) cgcgGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACgccgcc < 2:722774/80‑1 (MQ=255) cgGGTTCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTg > 2:722857/1‑81 (MQ=255) ggTTCACCCCTCCGTACCGCAGTTCGACCAGCCAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCa > 2:722865/1‑81 (MQ=255) tCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTa > 1:722823/1‑81 (MQ=255) tCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTa > 2:722853/1‑81 (MQ=255) tCACCACGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTa > 2:722858/1‑81 (MQ=255) accacGCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTAcc < 1:722775/81‑1 (MQ=255) gCCGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATa < 1:722776/81‑1 (MQ=255) ccGTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAg > 2:722856/1‑81 (MQ=255) gTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTc < 2:722778/81‑1 (MQ=255) gTACAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTc < 2:722777/81‑1 (MQ=255) aCAGCAGTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTcgc > 1:722873/1‑81 (MQ=255) cagcagTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTcgcg < 1:722779/81‑1 (MQ=255) gcagTTCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTCgcggc > 1:722834/1‑81 (MQ=255) | TATTTTCAGTAACCTGTGACATGATGTCCCCTTACTTCTTATGACGAATACGCGGGTTCACCACGCCGTACAGCAGATCGACCAGCAAGTTGACGAGGATAATCATCGTCGCCACCAGCAATACGCCGCCCTGCACTACCGGATAGTCGCGGC > NC_000913/3704740‑3704892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |