Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,708,650	T→C	noncoding (43/77 nt)	proK ←	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,708,650	0	T	C	100.0%	108.1 / NA	33	noncoding (43/77 nt)	proK	tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

CAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCT  >  NC_000913/3708574‑3708727                                                                             |                                                                              cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTc                                                                             <  1:723834/79‑1 (MQ=255) cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTc                                                                             <  1:723833/79‑1 (MQ=255)  aGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTc                                                                          <  1:723835/81‑1 (MQ=255)      gCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAGTCGAACCTCCGACCCCCTCGTCCcg                                                                       >  1:723878/1‑80 (MQ=255)       cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcgaa                                                                     <  1:723837/81‑1 (MQ=255)       cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcga                                                                      <  1:723838/80‑1 (MQ=255)       cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcga                                                                      <  2:723836/80‑1 (MQ=255)        aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcgaac                                                                    <  1:723839/81‑1 (MQ=255)          aaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcgaacga                                                                  <  2:723840/81‑1 (MQ=255)               ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTgc                                                             <  1:723841/81‑1 (MQ=255)                   cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTa                                                         >  1:723898/1‑81 (MQ=255)                     cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTAc                                                        >  2:723887/1‑80 (MQ=255)                          aGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCt                                                  >  2:723894/1‑81 (MQ=255)                          aGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCt                                                  <  2:723842/81‑1 (MQ=255)                            cAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgc                                                >  1:723896/1‑81 (MQ=255)                            cAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgc                                                <  1:723843/81‑1 (MQ=255)                             aGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg                                               >  2:723903/1‑81 (MQ=255)                              ggCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgc                                              >  2:723910/1‑81 (MQ=255)                               gCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgc                                              >  1:723899/1‑80 (MQ=255)                               gCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGcc                                             <  1:723844/81‑1 (MQ=255)                                      gAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                      <  1:723845/81‑1 (MQ=255)                                            ggTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGc                                >  2:723893/1‑81 (MQ=255)                                              tCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCgg                              >  2:723889/1‑81 (MQ=255)                                                ggTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCgggg                            >  2:723881/1‑81 (MQ=255)                                                  tGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgcg                          >  1:723897/1‑81 (MQ=255)                                                     tAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCAt                       <  1:723846/81‑1 (MQ=255)                                                      agagGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATc                      >  1:723906/1‑81 (MQ=255)                                                        agGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCtt                    <  2:723847/81‑1 (MQ=255)                                                            ttCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg                >  1:723892/1‑81 (MQ=255)                                                              cGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCGATCACCGAATGCGGGGCGCATCTTACTgc               <  2:723848/80‑1 (MQ=255)                                                                aaCCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcg              >  1:723895/1‑79 (MQ=255)                                                                   cTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGAt         <  1:723849/81‑1 (MQ=255)                                                                      cGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACg      >  2:723907/1‑81 (MQ=255)                                                                          ccccTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCt  >  2:723879/1‑81 (MQ=255)                                                                             |                                                                              CAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCT  >  NC_000913/3708574‑3708727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑