Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,538	G→T	intergenic (‑63/+261)	eptB ← / ← yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,538	0	G	T	100.0%	86.2 / NA	25	intergenic (‑63/+261)	eptB/yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/13);  total (12/13)

TTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTG  >  NC_000913/3710462‑3710613                                                                             |                                                                            ttGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGaa                                                                          >  2:724376/1‑80 (MQ=255) ttGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGaa                                                                          >  2:724365/1‑80 (MQ=255)          ctcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCg                                                                  >  2:724371/1‑79 (MQ=255)             aTGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCgg                                                                 <  1:724337/77‑1 (MQ=255)              tGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTa                                                            <  1:724338/81‑1 (MQ=255)                 aaacaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGt                                                          >  2:724374/1‑80 (MQ=255)                  aacaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGt                                                          >  1:724368/1‑79 (MQ=255)                  aacaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCa                                                        <  2:724340/81‑1 (MQ=255)                  aacaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCa                                                        <  2:724339/81‑1 (MQ=255)                   acaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGt                                                          >  2:724373/1‑78 (MQ=255)                          cTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTa                                                <  2:724341/81‑1 (MQ=255)                                          tAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGa                                <  1:724342/81‑1 (MQ=255)                                                ccTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGt                          <  2:724343/81‑1 (MQ=255)                                                 cTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAg                           >  1:724375/1‑79 (MQ=255)                                                 cTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAg                           >  2:724379/1‑79 (MQ=255)                                                    cTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAg                        <  1:724344/79‑1 (MQ=255)                                                     ttAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGAc                      >  1:724372/1‑80 (MQ=255)                                                        gTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACcac                   <  1:724345/80‑1 (MQ=255)                                                            cTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCa               >  2:724384/1‑80 (MQ=255)                                                               ttCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCaaa             <  2:724346/79‑1 (MQ=255)                                                                  aGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGa         <  1:724347/80‑1 (MQ=255)                                                                   ggCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGaaa       <  1:724349/81‑1 (MQ=255)                                                                   ggCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGaaa       <  2:724348/81‑1 (MQ=255)                                                                       cGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAgt   >  1:724387/1‑81 (MQ=255)                                                                        ggCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAgtg  >  2:724378/1‑81 (MQ=255)                                                                             |                                                                            TTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTG  >  NC_000913/3710462‑3710613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑