Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,170	T→C	N199S (AAT→AGT)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,170	0	T	C	100.0%	153.9 / NA	44	N199S (AAT→AGT)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

ATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCAT  >  NC_000913/3730098‑3730246                                                                         |                                                                             aTTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGc                                                                      <  2:729282/81‑1 (MQ=255) aTTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGc                                                                      >  1:729337/1‑81 (MQ=255)     tatgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGttt                                                                  >  2:729341/1‑81 (MQ=255)      atgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGttt                                                                  <  1:729283/80‑1 (MQ=255)       tgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCg                                                                <  2:729285/81‑1 (MQ=255)       tgtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCg                                                                <  2:729284/81‑1 (MQ=255)        gtttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCg                                                                >  1:729354/1‑80 (MQ=255)         tttatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTa                                                              <  2:729286/81‑1 (MQ=255)           tatgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAAc                                                            >  2:729360/1‑81 (MQ=255)            atgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAAcc                                                           >  2:729358/1‑81 (MQ=255)             tgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCt                                                          <  1:729288/81‑1 (MQ=255)             tgCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCt                                                          <  2:729287/81‑1 (MQ=255)               cTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCtt                                                         <  1:729291/80‑1 (MQ=255)               cTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTc                                                        <  1:729290/81‑1 (MQ=255)               cTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTc                                                        <  2:729289/81‑1 (MQ=255)                  aaCCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCAc                                                      >  1:729355/1‑80 (MQ=255)                   accaccATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGa                                                    >  2:729363/1‑81 (MQ=255)                    ccaccaTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGAc                                                   >  2:729364/1‑81 (MQ=255)                     caccaTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGAcc                                                  >  1:729353/1‑81 (MQ=255)                      accaTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCg                                                 >  1:729345/1‑81 (MQ=255)                        caTCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGcc                                               <  2:729292/81‑1 (MQ=255)                           cTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                            >  1:729351/1‑81 (MQ=255)                           cTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                            >  2:729365/1‑81 (MQ=255)                              cATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCca                                            <  2:729293/78‑1 (MQ=255)                                 aaaGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGa                                       >  2:729347/1‑80 (MQ=255)                                 aaaGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGa                                       >  1:729348/1‑80 (MQ=255)                                    gCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATa                                   <  2:729294/81‑1 (MQ=255)                                     cGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAg                                  <  1:729295/81‑1 (MQ=255)                                     cGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAg                                  <  2:729296/81‑1 (MQ=255)                                        cccAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGttt                               >  2:729350/1‑81 (MQ=255)                                         ccAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGtttt                              <  1:729297/81‑1 (MQ=255)                                          cAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTc                             <  1:729299/81‑1 (MQ=255)                                          cAGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTc                             <  1:729298/81‑1 (MQ=255)                                           aGTTGTTCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCa                            <  1:729300/81‑1 (MQ=255)                                              tgttCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCg                         >  1:729352/1‑81 (MQ=255)                                                ttCACGCTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGcc                       <  1:729301/81‑1 (MQ=255)                                                      cTCCTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAAttt                 <  2:729302/81‑1 (MQ=255)                                                        ccTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTAt               >  2:729357/1‑81 (MQ=255)                                                        ccTGACGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTAt               >  1:729374/1‑81 (MQ=255)                                                             cGCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGtt          >  2:729375/1‑81 (MQ=255)                                                              gCAAGTCGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTg         >  1:729372/1‑81 (MQ=255)                                                                    cGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCa   >  1:729389/1‑81 (MQ=255)                                                                    cGGTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCa   >  2:729378/1‑81 (MQ=255)                                                                     ggTACTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCAt  >  2:729373/1‑81 (MQ=255)                                                                         |                                                                             ATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACGACCGCCCCACAGGACATAGTTTTCACCGCCCAATTTATGGGTTGCTTCCAT  >  NC_000913/3730098‑3730246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑