Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,752,105	C→T	S38S (AGC→AGT)	yiaU →	putative LysR‑type DNA‑binding transcriptional regulator YiaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,752,105	0	C	T	100.0%	26.3 / NA	9	S38S (AGC→AGT)	yiaU	putative LysR‑type DNA‑binding transcriptional regulator YiaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/0);  total (9/0)

ATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACC  >  NC_000913/3752028‑3752182                                                                              |                                                                              aTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTaaa                                                                            >  1:735028/1‑81 (MQ=255) aTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTaaa                                                                            >  2:735029/1‑81 (MQ=255)  ttATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTaaa                                                                            >  2:735025/1‑80 (MQ=255)     tctcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTAAATATc                                                                        >  1:735032/1‑81 (MQ=255)         ggTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTAAATATCTTGc                                                                    >  1:735036/1‑81 (MQ=255)                               atCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGTAAATATCTTGCTGATTTTGAATCAAAAGTGGGt                                              >  2:735034/1‑81 (MQ=255)                                            cgACTGTACTTGGCATCGCACAGGCCAACGTCAGTAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCttt                                  >  1:735026/1‑80 (MQ=255)                                                       ggCATCGCACAGGCCAACGTCAGTAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccacc                      >  1:735035/1‑81 (MQ=255)                                                                           cAGTAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACAcc  >  2:735049/1‑81 (MQ=255)                                                                              |                                                                              ATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACC  >  NC_000913/3752028‑3752182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑