Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,757	G→C	A277P (GCG→CCG)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,757	0	G	C	100.0%	182.8 / NA	53	A277P (GCG→CCG)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/22);  total (31/22)

AGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGC  >  NC_000913/3795680‑3795833                                                                              |                                                                             aGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccgg                                                                           <  2:746608/81‑1 (MQ=255)  gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                          <  1:746609/81‑1 (MQ=255)   cTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggcg                                                                         <  1:746610/81‑1 (MQ=255)   cTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggcg                                                                         >  2:746694/1‑81 (MQ=255)    ttGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGgcgc                                                                        >  1:746699/1‑81 (MQ=255)      gATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCt                                                                       >  2:746691/1‑80 (MQ=255)       aTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTc                                                                      <  1:746612/80‑1 (MQ=255)       aTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTc                                                                      <  2:746611/80‑1 (MQ=255)        tCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCaa                                                                    >  2:746709/1‑81 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAGCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  >  2:746641/1‑81 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAAt                                                                   <  1:746615/80‑1 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  >  1:746705/1‑81 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  >  2:746693/1‑81 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  <  1:746613/81‑1 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  <  1:746614/81‑1 (MQ=255)          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  <  1:746616/81‑1 (MQ=255)           aGCGGTTATCCTGATTGCAGCCTGTAAAGCCAATGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCg                                                                 >  1:746683/1‑81 (MQ=255)              ggTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTcc                                                              >  2:746688/1‑81 (MQ=255)                  aTCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTg                                                          >  1:746702/1‑81 (MQ=255)                   tCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTg                                                          <  1:746617/80‑1 (MQ=255)                    ccTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGt                                                        >  1:746675/1‑81 (MQ=255)                      tGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTg                                                      >  2:746681/1‑81 (MQ=255)                       gATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGc                                                     >  1:746713/1‑81 (MQ=255)                        aTTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTg                                                      <  2:746619/79‑1 (MQ=255)                         ttGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGccc                                                   <  1:746620/81‑1 (MQ=255)                            cAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTg                                                 <  2:746621/80‑1 (MQ=255)                            cAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGt                                                <  1:746622/81‑1 (MQ=255)                             aGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTa                                               >  2:746656/1‑81 (MQ=255)                              gCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCCTGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTAt                                              >  1:746651/1‑81 (MQ=255)                               ccTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATg                                             <  1:746623/81‑1 (MQ=255)                                cTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATgg                                            >  1:746689/1‑81 (MQ=255)                                cTGTAAAGCCATTGTCACTAACGATGCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATgg                                            <  1:746624/81‑1 (MQ=255)                                  gTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTc                                          >  2:746700/1‑81 (MQ=255)                                  gTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTc                                          >  2:746684/1‑81 (MQ=255)                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTcc                                         <  2:746628/81‑1 (MQ=255)                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTc                                          <  1:746625/80‑1 (MQ=255)                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTc                                          <  2:746627/80‑1 (MQ=255)                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTc                                          <  1:746626/80‑1 (MQ=255)                                         cATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAg                                   <  1:746629/81‑1 (MQ=255)                                          aTTGTCACTAACGTTTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGc                                  >  2:746695/1‑81 (MQ=255)                                           ttGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGcc                                 >  1:746710/1‑81 (MQ=255)                                             gTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCg                               >  2:746686/1‑81 (MQ=255)                                                   aaCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACtt                          >  1:746690/1‑80 (MQ=255)                                                    aCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGACCGAGTAGCCCGGACTTc                         <  1:746630/80‑1 (MQ=255)                                                      gATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGTCCTGTATGGTCCGAGTAGCCCGGACTTcaca                      >  2:746692/1‑81 (MQ=255)                                                         tCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccg                   >  2:746698/1‑81 (MQ=255)                                                         tCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccg                   >  1:746706/1‑81 (MQ=255)                                                          cTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgc                  >  2:746707/1‑81 (MQ=255)                                                           tGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc                 >  1:746708/1‑81 (MQ=255)                                                               cTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTa             >  2:746719/1‑81 (MQ=255)                                                                tGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTAt            >  2:746722/1‑81 (MQ=255)                                                                 gATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATc           >  1:746701/1‑81 (MQ=255)                                                                  aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATc           <  1:746631/80‑1 (MQ=255)                                                                     cATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAt       <  2:746632/81‑1 (MQ=255)                                                                        gTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATa      >  2:746685/1‑79 (MQ=255)                                                                        gTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATa      >  2:746744/1‑79 (MQ=255)                                                                         ttGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAg   <  1:746633/81‑1 (MQ=255)                                                                           gcgccggcgCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAgc  <  1:746634/80‑1 (MQ=255)                                                                              |                                                                             AGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGC  >  NC_000913/3795680‑3795833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑