Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,839,737	A→G	V86A (GTC→GCC)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,839,737	0	A	G	100.0%	123.4 / NA	36	V86A (GTC→GCC)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/15);  total (21/15)

ACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCAACATCG  >  NC_000913/3839663‑3839814                                                                           |                                                                              aCGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCg                                                                         <  1:757121/81‑1 (MQ=255)   gTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCa                                                                       >  1:757152/1‑81 (MQ=255)      tttCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATc                                                                     <  2:757123/80‑1 (MQ=255)      tttCCCACCGCTACCAGTTTATAACCATGCACCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATc                                                                     <  2:757122/80‑1 (MQ=255)       ttCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATc                                                                     >  1:757151/1‑79 (MQ=255)          ccACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGt                                                                 <  1:757124/80‑1 (MQ=255)            aCCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCg                                                              <  2:757125/81‑1 (MQ=255)               gCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCa                                                           >  2:757166/1‑81 (MQ=255)                   ccAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGAt                                                       <  1:757126/81‑1 (MQ=255)                        ttATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTc                                                  >  1:757165/1‑81 (MQ=255)                        ttATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTc                                                  >  1:757184/1‑81 (MQ=255)                         tataACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCg                                                 >  1:757169/1‑81 (MQ=255)                           taACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTTGc                                                <  2:757127/80‑1 (MQ=255)                             aCCATGCGCCTGATTATCTTGGTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCAt                                              <  1:757128/80‑1 (MQ=255)                                    gcCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCgg                                       >  1:757153/1‑80 (MQ=255)                                    gcCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGt                                      <  1:757130/81‑1 (MQ=255)                                    gcCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGt                                      <  2:757129/81‑1 (MQ=255)                                     ccTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTa                                     <  2:757131/81‑1 (MQ=255)                                       tGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAg                                    >  1:757172/1‑80 (MQ=255)                                         aTTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCa                                  <  1:757132/80‑1 (MQ=255)                                            aTCTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACg                               >  1:757167/1‑80 (MQ=255)                                              cTTGTTCAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAAc                            >  2:757174/1‑81 (MQ=255)                                                    cAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTa                       <  2:757133/80‑1 (MQ=255)                                                    cAAGAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTa                       >  1:757183/1‑80 (MQ=255)                                                       gAAAGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATc                   >  1:757180/1‑81 (MQ=255)                                                         aaGGGTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATcc                  >  1:757194/1‑80 (MQ=255)                                                           gggTCGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTAc               >  2:757170/1‑81 (MQ=255)                                                               cGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGt           >  1:757196/1‑81 (MQ=255)                                                               cGATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGt           >  2:757163/1‑81 (MQ=255)                                                                gATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGtt          >  1:757173/1‑81 (MQ=255)                                                                gATGCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGtt          >  2:757190/1‑81 (MQ=255)                                                                   gCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCaa       >  1:757162/1‑81 (MQ=255)                                                                   gCTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCaa       >  1:757160/1‑81 (MQ=255)                                                                    cTGGAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCAAc      <  2:757134/81‑1 (MQ=255)                                                                      ggAAGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCAACAt    >  2:757193/1‑81 (MQ=255)                                                                        aaGGCATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCAACATCg  <  1:757135/81‑1 (MQ=255)                                                                           |                                                                              ACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAACCGCTAAATCCTACCAGTTCAACATCG  >  NC_000913/3839663‑3839814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑