Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,855,981	T→C	G109G (GGA→GGG)	yidH ←	conserved inner membrane protein YidH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,855,981	0	T	C	100.0%	137.9 / NA	40	G109G (GGA→GGG)	yidH	conserved inner membrane protein YidH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/23);  total (17/23)

CCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCT  >  NC_000913/3855904‑3856054                                                                              |                                                                          ccAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGcccc                                                                        <  2:761680/81‑1 (MQ=255)   aGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCAt                                                                      <  2:761681/81‑1 (MQ=255)       aTGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACg                                                                  <  2:761682/81‑1 (MQ=255)        tgtgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGa                                                                 >  2:761743/1‑81 (MQ=255)         gtgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGAt                                                                >  1:761727/1‑81 (MQ=255)            cgcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCAc                                                             <  2:761683/81‑1 (MQ=255)                 cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGc                                                          <  1:761684/79‑1 (MQ=255)                  ggCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCAc                                                       >  1:761732/1‑81 (MQ=255)                   gCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACg                                                      <  1:761685/81‑1 (MQ=255)                     tGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGAc                                                    >  1:761739/1‑81 (MQ=255)                       cAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCGCCGCCACGAcc                                                   <  2:761686/80‑1 (MQ=255)                        aGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCAt                                                 <  2:761687/81‑1 (MQ=255)                          tCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCa                                               <  1:761689/81‑1 (MQ=255)                          tCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCa                                               <  1:761690/81‑1 (MQ=255)                          tCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCa                                               <  1:761688/81‑1 (MQ=255)                            cTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCaa                                              <  1:761691/80‑1 (MQ=255)                              gggTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAatat                                           >  2:761733/1‑81 (MQ=255)                              gggTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAatat                                           >  1:761741/1‑81 (MQ=255)                               ggTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAatat                                           <  1:761692/80‑1 (MQ=255)                               ggTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAatat                                           >  2:761756/1‑80 (MQ=255)                               ggTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAata                                            <  1:761693/79‑1 (MQ=255)                                 tCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTaa                                        <  2:761694/81‑1 (MQ=255)                                   ggCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAg                                       <  2:761695/80‑1 (MQ=255)                                     cAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCt                                     >  2:761747/1‑80 (MQ=255)                                       aTGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAAt                                  <  1:761697/81‑1 (MQ=255)                                       aTGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAAt                                  <  2:761696/81‑1 (MQ=255)                                           gTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGAt                               >  1:761764/1‑80 (MQ=255)                                           gTCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGAt                               >  1:761753/1‑80 (MQ=255)                                            tCTGGCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATta                             <  2:761698/81‑1 (MQ=255)                                                gCTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATtaata                          <  1:761699/80‑1 (MQ=255)                                                 cTTTGCGGCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAg                        <  1:761700/81‑1 (MQ=255)                                                       ggCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTg                  >  1:761745/1‑81 (MQ=255)                                                        gCTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTgg                 >  2:761755/1‑81 (MQ=255)                                                         cTATCCGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGgt                >  1:761749/1‑81 (MQ=255)                                                             ccGGCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAg            >  2:761751/1‑81 (MQ=255)                                                               ggCATACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAggg          >  2:761740/1‑81 (MQ=255)                                                                  aTACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAg       >  2:761742/1‑81 (MQ=255)                                                                   tACAACACCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAgg      >  2:761759/1‑81 (MQ=255)                                                                     caacaCCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGt     <  2:761701/80‑1 (MQ=255)                                                                       acacCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCct  <  2:761703/81‑1 (MQ=255)                                                                       acacCAGCCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCct  <  1:761702/81‑1 (MQ=255)                                                                              |                                                                          CCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCT  >  NC_000913/3855904‑3856054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑