Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,817	A→T	intergenic (‑214/‑82)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,817	0	A	T	100.0%	94.0 / NA	27	intergenic (‑214/‑82)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/15);  total (12/15)

GAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGA  >  NC_000913/3863750‑3863889                                                                    |                                                                         gagaTCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtt                                                             <  2:763789/81‑1 (MQ=255)      cTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtttttt                                                         <  1:833030/80‑1 (MQ=255)         cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAAt                                                     <  2:763791/81‑1 (MQ=255)         cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAAt                                                     <  2:763790/81‑1 (MQ=255)          gTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                    <  1:763792/81‑1 (MQ=255)          gTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                    <  2:763793/81‑1 (MQ=255)              tCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAt                                                 <  1:763794/80‑1 (MQ=255)               cgcgAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAtt                                                >  1:763859/1‑80 (MQ=255)                  gAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAg                                             >  2:763823/1‑80 (MQ=255)                  gAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGc                                            <  2:763796/81‑1 (MQ=255)                  gAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGc                                            <  2:763795/81‑1 (MQ=255)                   aaaaaGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAg                                             >  1:763851/1‑79 (MQ=255)                   aaaaaGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCa                                           <  2:763797/81‑1 (MQ=255)                       aGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAAc                                       >  1:763831/1‑81 (MQ=255)                                ctGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTgcg                              >  2:763839/1‑81 (MQ=255)                                   tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCAt                           >  2:763827/1‑81 (MQ=255)                                    gAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCAt                           >  1:763821/1‑80 (MQ=255)                                     aGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTa                         <  1:763798/81‑1 (MQ=255)                                     aGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTa                         >  2:763830/1‑81 (MQ=255)                                       cacaGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAcc                       >  1:763834/1‑81 (MQ=255)                                           ggTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCggg                   <  1:763799/81‑1 (MQ=255)                                               cacaGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCtt                <  1:763800/80‑1 (MQ=255)                                                     aTCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGc          >  2:763845/1‑80 (MQ=255)                                                         aaCACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaa      >  2:763826/1‑80 (MQ=255)                                                          acacATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaaaa    <  2:763801/81‑1 (MQ=255)                                                          acacATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaa      >  1:763828/1‑79 (MQ=255)                                                            acaTCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAaga  <  1:763802/81‑1 (MQ=255)                                                                    |                                                                         GAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGA  >  NC_000913/3863750‑3863889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑