Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,876,270	A→G	I228T (ATT→ACT)	yidA ←	sugar phosphatase YidA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,876,270	0	A	G	100.0%	129.6 / NA	37	I228T (ATT→ACT)	yidA	sugar phosphatase YidA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/14);  total (23/14)

GTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGTGAT  >  NC_000913/3876198‑3876347                                                                         |                                                                              gTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGc                                                                        <  2:767205/80‑1 (MQ=255)   gACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGa                                                                      >  2:767258/1‑80 (MQ=255)    aCAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGata                                                                    >  2:767253/1‑81 (MQ=255)            cGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTc                                                            >  1:767239/1‑81 (MQ=255)             gCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTc                                                            <  1:767206/80‑1 (MQ=255)               cACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCt                                                          >  2:767245/1‑80 (MQ=255)               cACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTg                                                         <  1:767208/81‑1 (MQ=255)               cACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTg                                                         <  2:767207/81‑1 (MQ=255)                aCTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGa                                                        <  2:767209/81‑1 (MQ=255)                 cttcttTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGat                                                       >  1:767252/1‑81 (MQ=255)                  ttcttTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatc                                                      >  1:767237/1‑81 (MQ=255)                       tCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaa                                                 >  1:767242/1‑81 (MQ=255)                        cACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaat                                                >  2:767255/1‑81 (MQ=255)                         aCTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaatc                                               >  1:767249/1‑81 (MQ=255)                         aCTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaatc                                               >  2:767260/1‑81 (MQ=255)                                   tAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGAttt                                     >  1:767254/1‑81 (MQ=255)                                      cGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATttctt                                  <  1:767210/81‑1 (MQ=255)                                        ttATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTcc                                >  2:767243/1‑81 (MQ=255)                                            ccATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGtt                            >  1:767240/1‑81 (MQ=255)                                             cATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGttt                           <  1:767211/81‑1 (MQ=255)                                             cATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGtt                            <  2:767212/80‑1 (MQ=255)                                              aTCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTa                          <  2:767213/81‑1 (MQ=255)                                               tCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTaa                         >  1:767248/1‑81 (MQ=255)                                                cGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTaa                         >  1:767246/1‑80 (MQ=255)                                                 gCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATa                       >  1:767263/1‑81 (MQ=255)                                                    acacCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATAcc                     >  1:767250/1‑80 (MQ=255)                                                      acCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTaa                  <  1:767214/81‑1 (MQ=255)                                                         gACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAacac               <  2:767215/81‑1 (MQ=255)                                                            gCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTc            >  1:767265/1‑81 (MQ=255)                                                            gCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTc            <  1:767216/81‑1 (MQ=255)                                                                 cATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGc         >  1:767261/1‑79 (MQ=255)                                                                   tatTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGt     >  1:767270/1‑81 (MQ=255)                                                                    atTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGt     >  2:767259/1‑80 (MQ=255)                                                                    atTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGt     >  2:767267/1‑80 (MQ=255)                                                                     ttCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGTg    <  1:767218/80‑1 (MQ=255)                                                                     ttCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGTGa   <  1:767217/81‑1 (MQ=255)                                                                      tCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGTGAt  >  2:767276/1‑81 (MQ=255)                                                                         |                                                                              GTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGTGAT  >  NC_000913/3876198‑3876347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑