Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,910,355	C→A	G15V (GGC→GTC)	pstC ←	phosphate ABC transporter membrane subunit PstC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,355	0	C	A	100.0%	146.9 / NA	41	G15V (GGC→GTC)	pstC	phosphate ABC transporter membrane subunit PstC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/21);  total (20/21)

AGAGAGACAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGCCCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAACGCGTTTACAAAGTC  >  NC_000913/3910277‑3910432                                                                               |                                                                              agagagACAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGacc                                                                             <  2:776572/81‑1 (MQ=255)  gagagaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCt                                                                            <  1:776574/81‑1 (MQ=255)   agagaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCtt                                                                           >  2:776631/1‑81 (MQ=255)    gagaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCttt                                                                          <  2:776575/81‑1 (MQ=255)    gagaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCttt                                                                          <  2:776576/81‑1 (MQ=255)    gagaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCttt                                                                          >  1:776634/1‑81 (MQ=255)      gaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCttttt                                                                        <  2:776577/81‑1 (MQ=255)      gaCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCtttt                                                                         >  2:776617/1‑80 (MQ=255)       aCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTa                                                                       <  2:776578/81‑1 (MQ=255)       aCAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTa                                                                       >  2:776636/1‑81 (MQ=255)                    ccAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTaa                                                          <  1:776579/81‑1 (MQ=255)                    ccAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTa                                                           >  2:776628/1‑80 (MQ=255)                      aaCATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTaaaa                                                        <  2:776580/81‑1 (MQ=255)                      aaCATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTaaaa                                                        <  1:776581/81‑1 (MQ=255)                         aTCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAg                                                       >  1:776643/1‑79 (MQ=255)                            aaTAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGc                                                  <  1:776582/81‑1 (MQ=255)                             aTAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCt                                                 <  1:776583/81‑1 (MQ=255)                              tAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCtt                                                >  2:776650/1‑81 (MQ=255)                              tAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCtt                                                >  1:776629/1‑81 (MQ=255)                               aGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTg                                               >  1:776633/1‑81 (MQ=255)                                        aGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGcc                                      <  1:776584/81‑1 (MQ=255)                                         gcgcCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCa                                     >  1:776640/1‑81 (MQ=255)                                          cgccgcCAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCAt                                    <  1:776585/81‑1 (MQ=255)                                            ccgccAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCAt                                    >  2:776645/1‑79 (MQ=255)                                             cgccAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAg                                 <  1:776586/81‑1 (MQ=255)                                               ccAGTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGt                                >  1:776651/1‑80 (MQ=255)                                                  gTTTTACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTAct                            >  1:776660/1‑81 (MQ=255)                                                   ttttACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTActc                           <  1:776587/81‑1 (MQ=255)                                                     ttACCAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTActct                          <  1:776588/80‑1 (MQ=255)                                                        ccAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAg                      >  1:776627/1‑81 (MQ=255)                                                        ccAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAg                      <  2:776589/81‑1 (MQ=255)                                                        ccAGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGATGCAGCCATAAGTTACTCTTCAg                      >  1:776644/1‑81 (MQ=255)                                                          aGCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGtt                    <  2:776590/81‑1 (MQ=255)                                                           gCACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGtt                    >  1:776641/1‑80 (MQ=255)                                                            cACGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTa                   >  1:776675/1‑80 (MQ=255)                                                              cGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAAc                <  2:776592/81‑1 (MQ=255)                                                              cGCTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAAc                <  2:776591/81‑1 (MQ=255)                                                                cTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAAcgc              <  2:776593/81‑1 (MQ=255)                                                                cTGAAAATTATGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAAcgc              >  2:776638/1‑81 (MQ=255)                                                                         aTGTCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAACGCGTTTACa       >  1:776654/1‑79 (MQ=255)                                                                            tCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAACGCGTTTACAAAGt   >  1:776656/1‑80 (MQ=255)                                                                            tCGACCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAACGCGTTTACAAAGTc  <  2:776594/81‑1 (MQ=255)                                                                               |                                                                              AGAGAGACAATAATGCCACCCAACATCAATAGCACAATCAGCGCCGCCAGTTTTACCAGCACGCTGAAAATTATGTCGCCCTTTTTACCCGGTGGGTTAAAAGCAGGCTTGGTTGCAGCCATAAGTTACTCTTCAGTTAAACGCGTTTACAAAGTC  >  NC_000913/3910277‑3910432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑